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Show A Structural Analysis of the Myotis Lucifugus Genome T*r*sa J.Tuan [Mario Capecchi, Matthew Hoekin} Departm*nt of Human ThisthesJS addresses the hypctheVu that a conserved set of genes with different expression levels. Icte I Hsr\ ar>d timi ng. yreld di^rse morph^ogy Mapping the gene order andosntent oF a morpnologi cally cfotinct rnamrnaUhe Irttle brown bat Myotis luciFugus^wiN p*ov"de us with theiools necessary lo eKperlmenially rnanipulate the Uat genorne usir^ modern modular biology lechniques. sequer>red. nothing is knov*n about 1 he ba1 sgeneconteM, chromosome K. To cha r^rterize 1 he bat gerxime^i chromosome number and moi photfl v hi hinh resolution bat I i Ekrt fi biobtisi cells were grown a nd harvested to ^ol Isct meta phase cell spreads The metaphase ¦ were1henu^dtog?neiate?karyo1ypethrciunhC banding. ^'^ h allovfl For correct V*ent"iicntion or" pair oF b?1 ^ h rornosornes. Chmca I h u rnan whsre chromosome palnis were mHVilly used to try to determine 1h? rjeneosntent oFihe bat. These paints resuhed in pos'rtrve "dentifr:ation oF individual human ^ h lornosorne pairs when used on h urrmn nretn phaw ^prends For FISH. however, ZCO F|&H, the use of human pror>es on tmt meta phaw sprendswa^not successful ma nen^rntmg a positive result. An alternative method currently r>emg pursued Is to pami hurnan metaplwsB spiPnids usmg oat whole ^hrornosorne probes. Bat pobes are ^reaied through mi c rod"»ssection or" sp^cilV bat chromosomPMd^ntilTed oy G bnndmgar>do?mparison with the high resolution ba1 knryoiype already generated. P£fl is used 1o arnphfy and fluoresceni hy Inb^l DNAsotbat it Fundcns nsni probe, 1hese painis will e^ntual hy be used on human metapnase cell spreads to delineate homologous regions r>etwepn 1 he human and bat genomes through ZOO FISH Ifluoresoence in situ hybrid izatranl aHgnment The syntemc map will reveal the gene conteM or"ea^h bat chromosome based on homologyioih^ human genome. fcftw} Tuufl MarioCopecchi |
