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Show ERE&EA 2^ High Resolution Optical Microscopy and Spectroscopy David Anderson (Jordan Gorton) Department of Physics, University of Utah We work wlih an appautuiitiat comhlnei elemerrts of reaHfeJd -¦ nun m opcn_al microscopy (NiDMX acomkc fore* rn ncioscopy CAf M|. ¦ n rluowictfiLe micio^opy In an efTorc 10 achieve very hlgti-resolurlon fl uoieii_em Imao^ Halnlypuc.ihtnitroii-Qpe funurtns by canning a small c^ntacf probe acfOBthe surfcc* of a ruteiblj meai jring rts copogrd phy and other properties Whenih'rs prate li Illumhated by a laser. H can Focus thel^tn wsrycighclyon lhe maierlal. whkh iid n '.juit a itortKent ieiponse l hai can be analyzed to produce Images Urclmaiel^ rt"e Sspt co dt hfe^ reioluibn on a w no fe-mote-c u fe length scale f<10 nn] h^rch though iensitirtty to rfcceci a w no fe riuoreaEnt molecule, all In a physiologically re leunt environ mem_ are working on chi-s 11 i -" my peiiona I effons have been In tneeaploratlon of meihodiof daca atqul^cion and anafysfa to achlevetfie ,j -m s^rakcmobe ratio far Irna^e^ fiomoui pankulai mkroitope 1 hli "rt a que^ion relaclwly Lnej:plon?d by oi her^roupi workjrg In the same field, and i ill -.. natoprodiKe cleaner more useful m^agei by helping ro ehmmai^ iome of [he background nolieihac He tend to observe I worked on l h"rt problem by piepanno a number of corn puier ilmulai fcni ohhe-phy*al r heit ilmulaibni appnx4niated data that we wouU concelraUysee. from our em_i ual miL iQicopt.ai: c»ln[ I could perform a 5erlet of different nolw-reducihon algorithms on these slrmMatkins to analvs the itrengi hi oF eadi In apidrtlorx I was a ble to Biptore the paramecei-apace of che system by varying different Inpujc variables-the anipH rl ucfe of cttclllathDn of the sea n n mg pic-be, for exam pfe-ard ob^rvmgihe effect on the im-a^e produced by the simulation The adua nca^e of using slmulatkinssuchatihew Is 1 hat we car perform a number operatkmson Images of arbl-1 rary a rrangemencs of material^ without needing to lake l he time and expense of ad ually using the apparatus We can 1 hen uw the I nformatran we have earned to refine our methods c-f daca analysis, and even to determine zercain phvEJcakonaams by combining the slmuLatlonswkh experi menial obwrvatkm H I 4* -3Wrwn (a -:_ *d rrmi Ltod 1 rr^ k d Mi fa 0 » Ch|K4ft vi^i WrwyLH*>«-P#r7.nL:n Atacfiitwm. (A. PMlCil ^JbT" >bl Ehmic J b*rtiKd ftlUK»nUri»*rf ¦1- uni 11 _h Vi- M--I ¦ . |