| OCR Text |
Show Activation of ERK1/2 Akt and S6 Kinase, but not PKC, Prior to Cardiac Hypertrophy in Spontaneously Hypertensive Rats Carlos Cueva^ Angelisa Smith* [Shane Lcflci. J, David Symons, Justin Carls tram. Thunder Ja I ill) Department of Nutrition, College of Health, University of Utah hypen icphy "rs [he letun <£ si iess on tne heart due to boon sudi ai hypertension r hit re iuhiirnl.....I > i.....enr of ihe myM^e^heart muscle teJfch erftne w..... Its i-" bey work ruhdei i p ilchihtiriLieaitof bb&d prtiidre Can: ¦ ¦ I¦, • ¦ ¦ ¦ i¦ I¦ •. ¦ ¦ I ¦ ¦• i.....I.......!¦¦ ^ie ikskcfcardlat arrriyihrnii rnyDCdrdul Infaralon iTiurt aCtsckX and fieflrt falljre LJndef- blu-i nrj-ti_ul ji paihwaytchrt regjlate cardlat li^penwphy li v\m I for iht degebprneni of drugs arJ iHerapie41 hai vhiI I help prevew and^uie hevtout studies i*p&n lhar Aki. excracelluldr re^utiud hlnasttbHKH^i Pioteln kinase t (PK.I £. ^ arid plkan regulaie cardldt riypenrDphy hbwevtfr l he role of W[C5 and 0fl In yk) remains. cfirtrawfilaLWe hypothealasd Akr jiid bl\Kl .'"2 d'.riVdClun. I ¦¦ n i i HE 'Xcuri In spared necuily h^penenilgerai iSHMi htanihefcireirfce onset of cardiac hypen itiphy Inimunotib&were Lsedt&deCerrrurw [u[dl ar>d phrtiphttyLaced <pj PRCa W L fi. t All. EflKl/2. FCKIjSd klnase. ar>d S6 rltDscfral pioceinon cardbc honic^enaies Trom 9-10 week c\d ^HR -;n=6^ ar>d Wlttar Kyoto ccncrola iV/Kt, n=6^_No cardiac hypertrophy existed at 1 his age Lhear1-tK<Jy we^ht ^HR 3 93±O 1C vt-WKtigizailllnspltecirgreaiermeananerlalpietsurelnShR^SHfi 1 b2±3 ut_ WKY116± 3 m rnh^ p^DJ 11, suggesrlncj an early phase oh he I'm ¦¦¦ 11 i» *¦- I ¦< I1 ai I Aki were slgnlficaM ry activated I n ShR vs. WK'r'Ip-ERKl^lrKireasedo?^. p-EPKl/J total ERKl ^2 increased 60^ p-Akc Increased 34^ p-Akt ¦ toial W increased69^ p^DOl^.p-PDKl. an upaream a^tlraic-ror Aki. was unchanged.PKCa. pll.^t, p-PKCa/pll. p-PKCe were urKhdngeiL ar>d p-PKCci was rwt dwectaHe Thr3S?phc^phorybtlonon p/0 56 Wnas^a key regulaior of Iranscrlpicnal control downstream of Akt, PDKl. and ER^ was Increased AM lp<MSl In SHft as «sSer2H/2M phc^phorybtlonof^6prc<elnonthe4^fi rlboscmalsubunlt I increased /Cftfcv p<0^4}. Owp da1a Indicate acclva l&n of p^O^kina^e medlaied transitional machinery in lhe SHfl heart prior ten he on^tdf cardac hyr»rtrophy rs likely duetoAkc and EflKl/2adlvatlon. and nocPrJ_ We conclude l hat PKX. may noc be required for cardiac hyper! roptiy I n che SHfl. |
