| OCR Text |
Show 68 here permit the highly accurate identification of proteins at extremely low levels (i.e. attomole to femtomole amounts injected onto the column) allowing for the identification of immunoprecipitated proteins with low abundance. immunoprecipitation-based approach, limitations possible strategy with any include the the detergent-based OAT-protein complexes from synaptosomes. Further studies formation extraction of the to this As artifactual of PPls resulting from (i.e., Y2H, co-localization, reciprocal immunoprecipitation, and functional studies) would be necessary to definitively identify protein-protein interactions and their role in the regulation of those proteins In conclusion, we have developed a method to immunoprecipitate the DAT from rat striatal synaptosomes and identify OAT-interacting protein by LC/MS/MS and Western blotting. This method could also be used to identify PPls of other membrane-associated and using other mechanisms avenues techniques. synaptosomal proteins or to confirm PPls identified Identifying additional PPls will provide insights into underlying OAT function and regulation and could represent new of research and novel therapeutic targets for OA-related disorders. |
