| OCR Text |
Show 36 Rectal temperatures were assessed at 1-h intervals beginning 30 min prior to the first saline or METH injection. Mean temperatures over the course of the experiments were determined. For METH-treated rats, only rats which achieved mean rectal temperatures greater than 38 'C over the were used for analysis. Rats were sacrificed were approved by the University of Utah Committee and were of the experiment course by decapitation. All procedures Institutional Animal Care and Use conducted in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. Drugs and chemicals. S-( -)- 3-Chloro-5-ethyl-N- [( 1-ethyl-2-pyrrolidinyl) methyl]- 6-hydroxy-2-methoxybenzamide (eticlopride) hydrochlodde and R( '* )-7Chloro-8-hydroxy-3- methyl-1-phenyl- 2,3,4,5-tetrahydro-1 H-3- benzazepine (SCH23390) hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO). (±) METH hydrochloride was supplied by Research Triangle Institute (Research Triangle Park, NC). Drugs doses were calculated as the free base. Drugs were dissolved in 0.9% saline vehide. Tissue preparation and Western blot analysis. Synaptosomal tissues were prepared as previously described (Baucum et aI., 2004). were dissected, homogenized in ice-cold 0.32 M sucrose, Bri,efly, striata pH 7.4, and centrifuged (80'0 x g, 12 min; 4 'C). Supernatants wer e centrifuged (22,000 x g, 15 min; 4 CC) and the resultant pellets were resuspended in ice-cold double distilled H20 at concentrations of 45-55 mg/ml original wet weight. Total protein concentrations were determined as described by Bradford (1976). The samples were then diluted with a nonreducing loading buffer (final concentration: 2.25% |
