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Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page27
Description THE MYSTERY OF DNA REPLICATION 27 commonly known as genetic engineering. Thus, if polymerase I is not the enzyme which replicates DNA, it has served a valuable purpose in opening a new field of research, the analysis of gene structure â€" an illustration that the pursuit of knowledge is an end in itself whose value is impossible to evaluate except in an historical context. In the early 1970's studies begun ten years earlier by Werner Arber resulted in the isolation of enzymes known as restriction enzymes. These cut DNA into large pieces with uneven ends. Since each particular enzyme always cuts DNA at a characteristic sequence of subunits, the overlapping strands of different pieces can pair. This allows pieces with these identical ends to be joined together. From this has developed the field now known as genetic engineering: the ability to join different pieces of DNA together and then to replicate the new genome. Another use of these tools has been the ability to analyze the structure of particular genes or of many genes. To do this, genes have been cut apart into recognizable pieces; these pieces have been copied with radioactive subunits and these radioactive pieces used to identify specific parts of the chromosome containing similar pieces. Furthermore, the order of subunits can be determined in each radioactive piece so that in fact the structure of DNA now can be known in complete detail down to the ordering of the smallest subunits. From such studies we are beginning to realize that the structure of genetic material is quite complicated: there are large regions of DNA in which the order of subunits is highly repetitive, or the sequence is repeated many times. It is not clear what these regions do, except that (so far) they do not appear to function as genes (that is, they do not determine a characteristic of the cell; they are not translated into proteins). The number of copies of particular genes also can vary and we are beginning to believe that one way in which inheritance can be adapted to deal with new environments may be to change the number of copies of individual genes rather than the information within single genes. Another new characteristic is that some genes are found to change position within
Format application/pdf
Identifier 033-RNLT-LarKK_Page27.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319388
Reference URL