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Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page15
Description THE MYSTERY OF DNA REPLICATION 15 replication. One regulated initiation, the other regulated DNA synthesis which had already begun. The latter was not surprising since it could be assumed that the mutation had changed the polymerase I enzyme, the protein that made DNA. However, the fact that initiation could be changed by mutation as well indicated that this, also, was regulated by some protein. At the same time, Ole Maaloe, with whom I had worked in Denmark, obtained data suggesting that the cell had to make a new protein on each occasion that it initiated DNA replication. Until then, this had never been observed in studies of regulation and indeed this seemed absurd, because the Paris group had shown that "old proteins never die; they just fade away." That is, protein synthesis could stop but proteins already present in cells remained active and continued to function in daughter cells into which they were distributed. This new hypothesis implied that proteins necessary for DNA synthesis were either destroyed or could not be reused and had to be resynthesized with each cell cycle (in bacteria, every half hour). Because of my prejudice against this waste, or perhaps in rebellion against my former mentor, I set out to prove that this idea was wrong, only to succeed in demonstrating conclusively that my mentor was correct. DNA replication always began from the same unique point on the molecule and proceeded sequentially until the entire chromosome (or DNA molecule) was completed, but each cycle of replication did indeed require synthesis of new protein. These experiments, carried out with living cells, did not contradict the in vitro experiments with enzymes. But they indicated that initiation of replication might be a new field of study which enzym-ology had not yet touched. Curious about this problem, geneticists and cell biologists began to dig into this area to unearth new phenomena. As these came to light it became clear that DNA replication could not be as simple as had been believed. Some of the in vivo results even appeared to be in direct conflict with the enzymology of DNA replication. Pursuing the fate of proteins needed to initiate DNA replication, we found that not one, but several, were required and that each of these was made at a different time somewhat before DNA replica-
Format application/pdf
Identifier 021-RNLT-LarKK_Page15.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319376
Reference URL