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Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page24
Description 24 KARL G. LARK thesis was reused many times, portions of it might begin to wear out or become faulty and replication could produce mistakes. By synthesizing the replication machinery anew it is assured that it will be as accurate as possible. Why do we have so many interacting components? We can only speculate about this. We already know that a single enzyme can do the job, but only using a DNA template which has already been unwound and only if it is provided with a free end to which it can add new subunits. Different proteins may be required to unwind the DNA and to provide starting ends. Moreover, if a replication protein were made which was faulty, it would generate a tremendous quantity of mutations (possibly lethal mutations) in the DNA that is synthesized. On the other hand, if such a protein has to fit into the context of many other proteins and their surfaces all have to be precisely joined, the part must be precisely machined. Most defective components would not fit into the complex so replication would not start but would wait until a new replication machine has been assembled that could fit together properly. The polymerase which has to act successfully may be situated somewhere in the core of this complex in such a way that it cannot possibly function well unless it is within the context of all the other protein surfaces which assure that it is the right shape and configuration to do its job perfectly. An exception to this rule is a special complex which can be demonstrated in bacteria. This complex can be reused to replicate DNA again and again but is less accurate and makes many mistakes or mutations. At first one might conclude that this is bad for the cell, but bacteria have used this situation to their advantage. In nature, bacteria live in a variety of environments: sometimes in rich surroundings complete with all of the nutrients they need and sometimes in environments poor in foodstuffs. In the latter, they have to synthesize nutrients themselves and often may lack the genetic capability to enable them to accomplish their life struggle successfully. Without the ability to change, the bacteria cannot survive. One way in which bacteria insure that they can improve their ability to adapt to new environments is to make replication machines
Format application/pdf
Identifier 030-RNLT-LarKK_Page24.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319385
Reference URL