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Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page26
Description 26 KARL G. LARK good reason for its existence and that understanding the process within the context of the organization of a cell will lead to an understanding of why it is suited to the survival of the organism. The attraction of the Watson-Crick model was the promise of an elegantly simple process for duplicating genetic information. In fact, the process has turned out to be absurdly complicated. The product does not appear as a simply polymerized elongating chain of subunits. Instead, the templates are copied in little pieces which are finally joined together. Either there is no other way in which DNA can be replicated or there must be a purpose to discontinuous synthesis which we do not yet understand. To speculate about this, we must review other advances in the area of genetics and inheritance which are related to DNA replication. At no other time in the history of biology has the structure of the heritable material been under such intense scrutiny as it is today. This is, in part, a legacy of the early advances in the enzym-ology of DNA replication and, in part, the result of a separate development in genetic molecular biology: the study of enzymes (restriction enzymes) which specifically destroy DNA. The early studies of DNA enzymes led to the development of techniques to isolate, characterize, and use enzymes which elongate DNA chains, such as polymerase I, or join pieces of DNA together such as the DNA joining enzyme. The work established general principles for the characterization of new enzymes which interact with and specifically cut DNA into pieces. Perhaps the most important outcome of this period of research was the training of two generations of biochemists in completely new, very sophisticated approaches to enzymes and DNA. In the studies of polymerase I and later, in the studies of discontinuous synthesis, ingenious techniques were developed to manipulate DNA, to fill in gaps with radioactive subunits, to join separate pieces to each other. All of these developments were the result of Arthur Kornberg's efforts to unravel the mysteries of polymerase I and its related enzymes. He and his collaborators developed a new approach to the enzymological manipulation of nucleic acids which has subsequently been adopted for most of the modern approaches to nucleic acid structure, a field
Format application/pdf
Identifier 032-RNLT-LarKK_Page26.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319387
Reference URL