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Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page6
Description 6 KARL G. LARK biochemists: (a) how is the unwinding process carried out and can we prove that this is how the two copies of DNA are made? (b) how is the DNA double helix used to create the variety of protein molecules (the chemical machines) which run the cell? At the end of that seminar I left for Denmark and later Geneva to do postdoctoral research in laboratories of scientists who were part of the phage group. Although there were no immediate experimental consequences of the Watson-Crick model, everybody was now agreed that DNA was the genetic material. A lot of thinking was going on and experimenting was beginning. Looking back, I realize that the stage was being set for one of the most exciting decades in the history of any science. At the 1953 Cold Spring Harbor Symposium, Bill Hayes had clarified many of the puzzling properties of mating in the bacterium, E. coli (in fact demonstrating the existence of male and female bacteria), and thus had made Joshua Lederberg's discovery of bacterial mating generally useful. This, together with a new system of genetic transfer (transduction), also developed in Lederberg's laboratory by Norton Zinder, had turned this bacterium into one of the most useful genetic systems ever known. This organism and its viruses now became the system of choice for the study of biochemical problems and was being used extensively by the Paris group. (The problem of adaptive growth in bacteria had been translated into the regulation of protein synthesis by Jacques Monod and the genetics of this system were being studied by Frangois Jacob.) Seymour Benzer had devised an ingenious genetic system using phage with which he defined the limits of length of a gene, but whose general application opened new avenues for exploring the interactions of genetic systems. During these three years a revolution in genetic techniques and thinking occurred. A revolution in scientific technology also occurred as one of the few fringe benefits of the nuclear age. As a graduate student, I had used radioactive isotopes only once. Now, isotopes became a way of life. 32P and 35S were readily available and these could be used to label DNA or protein, respectively. Two much more useful isotopes 3H and 14C, were about to become available. The electron
Format application/pdf
Identifier 012-RNLT-LarKK_Page6.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319367
Reference URL