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Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page21
Description THE MYSTERY OF DNA REPLICATION 21 a self-portrait of replicating chromosomes from animal cells. Unlike bacteria, these cells duplicate the single, huge DNA molecule which is a single one of their many chromosomes by individually replicating several hundred regions of the large molecule. Since not all of these regions are replicated at the same time, the DNA molecule may contain portions which have replicated but have to wait for intervening regions to be replicated before all of the new pieces can join to give two daughter chromosomes. This proved to be a universal pattern of DNA replication for cells of higher organisms, plant or animal. Somehow the joining is so ordered that old templates are almost always joined to old templates, instead of new DNA. Almost, but not always: occasionally a visible patch remains in which old DNA is joined to new. Almost twenty years had passed since the Watson-Crick model. Whereas the process of making proteins had been worked out and clarified, replication had passed from apparent simplicity into a complexity which boggled the mind. What factors were responsible for the difference? Certainly the two problems demanded different degrees of technical effort. The proteins which were studied in order to unravel the processes of transcription and translation were relatively abundant. Thus, genetics, biochemistry, and in vivo experiments could all be carried out in a single laboratory. On the other hand, each cell contained only a few molecules of the proteins involved in replication; they are essential to life and require herculean efforts to isolate. When attempts were made to isolate mutant replication proteins, these were difficult or impossible to obtain because either the mutant cells are too sick to grow well, or the proteins fall to pieces in the isolation procedures. Few laboratories could afford to study replication enzymes, and replication chemistry became separated from replication genetics and cell biology. The general acceptance of new observations depends to a large degree on the number of different scientists who can participate in these experiments. This is particularly important when the data are not easily fitted into a model or preconceived picture of nature. With DNA replication, scientists had begun with a simple model derived from the Watson-Crick structure and proceeded to uncover
Format application/pdf
Identifier 027-RNLT-LarKK_Page21.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319382
Reference URL