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Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page11
Description THE MYSTERY OF DNA REPLICATION 11 can divide to form new cells as often as every twenty minutes. Investigators learned how to manipulate them. Mating could be controlled so that small but precise bits of genetic material could be exchanged. In addition, viruses existed containing smaller segments of DNA which could be known in their entirety and whose proteins could be inventoried. All of this was based on the dogma that one string of information (linear) contains the entire blueprint of a cell. From what I have said already, it must be clear that one property of genetic information runs through all of this history of the early days of molecular biology. There is a continual emphasis that genes are units on a string, that these strings are long (in bacteria, one to two millimeters in length) and that the integrity of the string and the pattern of the gene's position in the string cannot be changed without changing the organism. Thus, there was a natural prejudice in the minds of most geneticists that the linear integrity of DNA could not be disturbed, that one use of the double helix was to protect that integrity. Cutting or breaking the double helix should be avoided. The very earliest models of replication consisted simply of unwinding the strands and progressively synthesizing copies along these unwound strands as fast as they were separated, thus protecting the single-strand templates. The Replication of DNA At first it seemed that the replication of DNA would be worked out before any other aspect of the central dogma. The striking complementarity of the double helix and its implication for the reproduction of genetic material raised this issue as the first critical test of the usefulness of the model. Elegant experiments were devised to test whether the integrity of the templates was maintained. In the first of these, it was shown with the new isotope 3H that when the DNA in a chromosome of a higher cell was duplicated, each of the two daughter chromosomes contained half of the old chromosome. After that, the half of the old chromosome which was used as a template was maintained intact and in
Format application/pdf
Identifier 017-RNLT-LarKK_Page11.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319372
Reference URL