Update item information
Title Mystery of DNA replication, The
Subject DNA--Synthesis
Description The 43rd Annual Frederick William Reynolds Lecture.
Creator Lark, Karl G.
Publisher University of Utah Press
Date 1980-03-05
Date Digital 2008-05-29
Type Text
Format application/pdf
Digitization Specifications Original scanned on Epson Expression 10000XL flatbed scanner and saved as 400 ppi uncompressed tiff. Display images generated in PhotoshopCS and uploaded into CONTENTdm Aquisition Station.
Resource Identifier,83
Source QP624 .L37
Language eng
Relation Digital reproduction of "The Mystery of DNA replication," J. Willard Marriott Library Special Collections
Rights Digital Image Copyright University of Utah
Metadata Cataloger Seungkeol Choe; Ken Rockwell
ARK ark:/87278/s65q4t2n
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-08-04
ID 319398
Reference URL

Page Metadata

Title Page14
Description 14 KARL G. LARK make exactly the right choice. Indeed this first demonstration of the opposite polarity of the two strands also demonstrated the correctness of the Watson-Crick structure and the power of enzym-ology as a tool in studying structure. Since this experiment used Kornberg's DNA-synthesizing enzyme, it was felt that the experiment represented almost conclusive proof that this enzyme was responsible for copying DNA in the cell. This conclusion was further strengthened by the demonstration that the enzyme made virtually no mistakes (that is, inserting incorrect subunits in place of the correct ones). This, of course, was essential for the enzyme which copies DNA since we know that mutations are very rare events. Indeed, if mutations occurred too frequently they would destroy the integrity of the cell, for the mutant proteins that would be niade would not perform their proper function. The accuracy with which the enzyme copied DNA was phenomenal, less than one mistake per million subunits. Thus, within seven years after the proposal of the double helix, the essential features of DNA replication seemed to have been confirmed. Somehow the replication was begun and thereafter the polymerase copied the templates % the molecule unwound. In 1956 I began an academic career of research and teaching. During the next seven years, which I spent in St. Louis University, I pursued the problem of "what ciUSes cells to divide?" Eventually, this became the problem of "what initiates DNA synthesis in a cell?" Recent experiments had s^wn that most cells which did not divide also somehow had stop^d replicating their DNA. When division was to be resumed, the first cellular act always was to copy the DNA. If initiating DNA replication committed the cell to a sequence of events leading to division, what determined initiation of replication? Although more was becoming known about DNA replication, little of this knowledge helped t0 answer or even approach the problem of initiation. Finally, after almost seven years, some progress was made in this area: Following the genetic approach which had been so successful for the regulation of protein synthesis, the Paris group had obtained two mutants which regulated DNA
Format application/pdf
Identifier 020-RNLT-LarKK_Page14.jpg
Source Original Manuscript: The mystery of DNA replication by Karl G. Lark.
Setname uu_fwrl
Date Created 2008-07-29
Date Modified 2008-07-29
ID 319375
Reference URL