| OCR Text |
Show 78 distribution. Participation in a single event guaranteed that the correlation coefficient would be high. The ROI correlation coefficient histogram skewness (R skewness) was calculated for each cell (Control: 1.503 ± 0.7738; LPS6: 1.592 ± 0.8902; LPS24: 1.202 ± 0.5642) as an approximate measure of event size. Significant differences in the median R skewness were detected (p < 0.0001, KW-statistic = 20.91; KW-ANOVA). The median R skewness of the LPS24 group was significantly lower than either Control or LPS6, whereas no difference was detected between the Control and LPS6 groups (Control vs. LPS6: p > 0.9999; Control vs. LPS24: p = 0.0003; LPS6 vs. LPS24: p = 0.0001; Dunn's Post-hoc test). Similarly, differences in the cumulative distribution of the LPS24 group R skewness were significantly left-shifted relative to Control or LPS6 (Control vs. LPS6: p = 0.89, D = 0.08; Control vs. LPS24: p = 0.0004, D = 0.27; LPS6 vs. LPS24: p = 0.0002; D = 0.30; KS-test; Figure 4.4D). High numbers of events prevented meaningful statistical analysis of intrinsic event characteristics. We observed no obvious physiologically relevant differences in mean ΔF/F0 (Control: 1.29 ± 1.29; LPS6: 1.34 ± 1.35; LPS24: 1.28 ± 1.30), mean duration (Control: 3.16 ± 1.68; LPS6: 3.32 ± 2.17; LPS24: 3.29 ± 2.14), or mean AUC (Control: 4.29 ± 3.93; LPS6: 4.94 ± 5.54; LPS24: 4.65 ± 4.77). Further analyses will need to be performed in order to detect differences in event characteristics. We also compared the sum of all event AUC values at each distance from the cell process boundaries (Figure 4.4C). By the 24-hr time point, the only observable events were located less than ~10 um away from the termini, and very little activity, if any, was observed near the soma (~30-35 um). It may be that intrinsic calcium activity in astrocytic processes is decreasing concentrically, from the soma and moving out towards the fine processes, during the acute phase of endotoxemia. |
