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Show 138 Sai Samineni school of medicine and health sciences Introduction: MicroRNAs are a class of single stranded RNAs that regulate gene expression by pairing with 3' untranslated regions of target messenger RNAs. Patients with cardiovascular disease have a correspond-ing dysregulation of miRNA expression. This study hypothesizes that the expression levels of pro-apoptotic (1 & 208a) and anti-apoptotic (133a/b & 21) miRNA in peripheral blood can be used as a diagnostic tool to monitor heart functionality in ischemic (IS) and idiopathic (ID) heart failure patients and the effects of any administered therapy measured with left ventricular ejection fraction (EF). Methods: 92 healthy controls (HC) with average EF>61% provided RNA for normal reference, 38 ID patients with EF<45%, and 36 IS patients with EF<45%. 6 ID and 4 IS patients were followed-up with consecutive blood draws. Samples were stored in Paxgene Blood RNA tubes which stabilize intracellular RNA. The miRNA was processed using PAXgene Blood miRNA Kit by PreAnalytiX. The RNA was quantitated with the Nano-Drop spectrophotometer to make 2ng/uL working samples. Five miRNA transcripts were quantified following reverse transcription and real-time polymerase chain reaction; expression levels were normal-ized to an internal control (U47). After testing for normality, group comparisons were performed using a t-test. Results: No age or gender associations were observed with any miRNA expression. Of the 5 miRNA tested, 4 were significantly associated with IS- HF, 5 with ID-HF and 5-with both (Table 1) Conclusions: • Several peripheral blood miRNAs are significantly dysregulated in both ID and IS patients when com-pared to normal controls. • Despite the measurability of peripheral blood miRNA levels with the ΔCT method, this study is limited by the lack of direct measurement such as the concentration of miRNA per unit of peripheral blood. • The study was also limited by a small number of follow-up patients with heart functionality data for consecutive blood draws. • Potential use of microRNA expression changes as a diagnostic/prognostic tool deserves further explora-tion on a larger group of patients before the current hypothesis can be analyzed more definitively. Table 1. MiRNAs Expression CIRCULATING miRNA AS A DIAGNOSTIC TOOL FOR ISCHEMIC AND IDIOPATHIC HEART FAILURE PATIENTS. Sai Samineni (John Carlquist) Department of Internal Medicine University of Utah UNDERGRADUATE RESEARCH ABSTRACTS John Carlquist Data expressed as a mean ± standard deviation dCT expression values; miR, micro-RNA; HC, healthy control; ID, idiopathic; IS, ischemic. MiRNA expression was analyzed for patients who improved with therapy or had no change or worsened with regard to their EF (Table 2). Data expressed as a mean ± standard deviation dCT expression values. |