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Show 132 Christopher D. Osborn school of medicine and health sciences Fibromyalgia Syndrome (FMS) and Chronic Fatigue Syndrome (CFS) are two medical conditions for which few remedies exist for patients combating these disorders. Because of this, research to advance current ac-ademic knowledge of the physiological factors associated with these diseases is needed. Previous studies have shown that multiple metabolite receptors work synergistically to activate key ion channels in mouse dorsal root ganglion (DRG) neurons that play a large part in development of fatigue and delayed muscle pain, the major symptoms of FMS and CFS. It is important to determine the percentage of each receptor on the various populations of DRG neurons within the DRG. Mouse DRGs can be used as they correlate well to of human DRGs. DRG's were taken from the lumbar region of mice spinal chords and cultured for 12 - 24 hours. From these cell cultures, individual cells were collected using a single cell collection procedure and receptors were studied using gene expression assays. The single cell collection process involved the use of a micromanipulator robot, the Meta Imaging Series MetaFlour software, and a precision micro-pipette system to accurately select and collect for neurons from the cell cultures. These individual cells were then analyzed using real-time PCR analysis of the neuronal mRNA to determine their gene expres-sion ratios that differ between cell types. The cell collection and PCR processes will be used to obtain data concerning the makeup of molecular cell surface receptors involved in pain and fatigue responses located on mouse DRGs. DISTRIBUTION OF MOLECULAR METABOLITE RECEPTORS ON DRGS IN MICE Christopher D. Osborn (Alan R. Light, Ron Hughen, E.C. Bang) UUSOM Department of Anesthesiology University of Utah UNDERGRADUATE RESEARCH ABSTRACTS Alan R. Light Ron Hughen E. C. Bang |