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Show HEALTH SCIENCES LEAP PROGRAM SPRING 2013 Leslie Florian Dustin L. Willams 158 DETERMINING THE ABILITY OF E+ POLYTO RESIST BACTERIAL ATTACHMENT Leslie Florian (Julia M. Lerdahl, Dustin L. Willams, Roy D. Bloebaum) Department of Orthopedics, Bone & Joint Laboratory University of Utah and Veterans Administration University of Utah Background: Biofilm related infections affect thousands of patients with total joint replacement every year. These infections can often lead to septic failure which can be health threatening and very costly due to revision surgery. In order to prevent biofilm infections from occurring, companies began to examine a variety of blended materials that can prevent the attachment and formation of bacteria to surfaces. In this study, a proprietary material known as E+ Poly was obtained from a company and tested for its ability to resist adherence of a clinical isolate of methicillin-resistant Staphylococcus aures (MRSA). Method: By utilizing Brain Heart Infusion (BHI), 10A5 MRSA cells were flowed through a flow cell system for a 48 hour period. Using scanning electron microscopy (SEM) an n=7 of five different sample were used to collect biofilm images; that had formed on the surface of each material type. Quantification was completed by vortexing, sonicating and using a tenfold dilution series plated on agar plates. Results: Based on the data obtained it appears as the E+ poly material did not have the ability to resist the formation of biofilms on the surface. SEM images support data that was obtained from quantification; presenting areas with biofilm formation in each material (Figure 1). Remarkably each of the five material showed very little or no bacteria formation (Figure 2). Conclusion: Biofilm infections can cause further complication with patient implants or even be life threatening if not treated immediately. The data specified that E+ poly combined materials is not an effective in preventing the growth of biofilms of a clinical isolated strain of MRSA. Although further research may be required, this study confirmed the ability of a clinically relevant isolate of M R S A to attach to and form biofilms on the surface of E+ poly materials as well as other material types that are clinically used. Figure 1: Representative S E M images collected from each of the five material types following 48 hours of incubation. Each materia] type had areas wherein bacteria attached to the surface as well as biofilms that formed. Figure 2: Representative S E M images collected from each of the five material types following 48 hours of incubation. These images show surface areas of each material type that had little to no bacterial attachmcnt'biofiLm formation. |
