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Show SCHOOL OF MEDICINE AND HEALTH SCIENCES UNDERGRADUATE RESEARCH ABSTRACTS Alexandra Butler Bryan Jones 112 RETINAL METABOLIC RESPONSES TO CIGARETTE SMOKE Alexandra Butler (Bryan Jones) Department of Ophthalmology University of Utah Purpose: Investigate effects of smoking on the retina, with regard to impacts for development of Age-related Macular Degeneration (AMD) by examining the Muller cells (MC) and retinal pigment epithelium (RPE). Effects of cigarette smoke on retinas of wild type (wt) mice and mice with either the alternative pathway (CfB) or the c o m m o n terminal pathway (C3) removed were examined. Methods: Mice were exposed to cigarette smoke (CS) or filtered air. CS was generated using an automated cigarette-smoking machine (Model TE-10, Teague Enterprises, Davis, CA) burning 3R4F reference cigarettes (2.45 m g nicotine per cigarette). Mice were exposed for 6 hours/day, 5 days/week for 6 months. Age matched filtered air exposed mice were controls. Eyes were enucleated immediately post-mortem, fixed in 1% paraformaldehyde, 2.5% glutaraldehyde, dehydrated in graded methanols, embedded in eponatesand histologically analyzed with CMP. Results: The RPE of smoked (s) WT mice showed slight increases in taurine (TT), glutathione (J), and decrease in glutamate (E) relative to non-smoked (ns) W T mice. The RPE of nsCfB knockout mice showed decreases in GABA (YY), E, glutamine (Q), J, argenine (R), and aspartate (D) relative to nsWT mice. The sCfB knockouts showed increases in R, and CRALBP, and a decrease in E relative to nsCfB mice. Overall, sCfB mice showed decreases in E, Q, J, R, and D when compared to nsWT mice. RPE in nsC3 knockouts showed decreases in YY, E,TT, Q, R, and D, and increases in CRALBP relative to n s W T sC3 had increased levels of YY, E, TT, J, R, and D, and decreased levels of CRALBP relative to nsC3 mice. Overall, levels of YY, TT, and J increased and levels of Q decreased in sC3 mice as compared to nsWT mice. The Muller cells (MC's) of sWT mice showed decreases in concentrations of YY, E,TT, Q, J, R, CRALBP, and glutamine synthase (GS) relative to nsWT mice. nsCfB MC's showed deceased levels of Q, R, D, CRALBP, and GS compared to nsWTmice. sCfB animals showed further decreases in levels ofYY, E,TT, J, D, andGS. Overall, when compared to nsWT mice, sCfB mice had dramatic across the board decreases in YY, E,TT, 0, J, R, D, CRALBP, and GS. MC's in nsC3 mice had decreased levels of YY, E,TT, Q, J, R, D, CRALBP, and GS relative to nsWTmice. SC3 mice had increased levels of YY,TT, Q, J, R, and CRALBP, and decreased levels of E compared to nsC3 mice. Overall sC3 mice showed decreases in E, Q, R, D, CRALBP, and GS, and increases in J relative to nsWT mice. Butler.1 - After clustering cell classes, Muller Cells (black) and RPE (white) were isolated for analysis |