| OCR Text |
Show 139 7. Inject sgRNA and Cas9 as described before (Shah et al., 2015). 8. Identify germline transmitted null mutation and fish carrier by HRMA and sequencing (Talbot and Amacher, 2014). References Bassett, A.R., Tibbit, C., Ponting, C.P., and Liu, J. (2013). Highly efficient targeted mutagenesis of Drosophila with the CRISPR / Cas9 System. Cell Rep. 4, 220-228. Doench, J.G., Hartenian, E., Graham, D.B., Tothova, Z., Hegde, M., Smith, I., Sullender, M., Ebert, B.L., Xavier, R.J., and Root, D.E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat. Biotechnol. 32. Hwang, W.Y., Fu, Y., Reyon, D., Maeder, M.L., Tsai, S.Q., Sander, J.D., Peterson, R.T., Yeh, J.J., and Joung, J.K. (2013). Efficient genome editing in zebrafish using a CRISPRCas system. Nat. Biotechnol. 31, 227-229. Montague, T.G., Cruz, J.M., Gagnon, J. a, Church, G.M., and Valen, E. (2014). CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res. 42, W401-7. Shah, A.N., Davey, C.F., Whitebirch, A.C., Miller, A.C., and Moens, C.B. (2015). Rapid reverse genetic screening using CRISPR in zebrafish. Nat. Methods 12, 535-540. Talbot, J.C., and Amacher, S.L. (2014). A Streamlined CRISPR pipeline to reliably generate zebrafish frameshifting alleles. Zebrafish 11, 583-585. |
