| OCR Text |
Show 49 Figure 3.2 Lef1 promotes neurogenesis in the zebrafish caudal hypothalamus (Hc). (A-C) Progressive reduction of Hc size in lef1 mutants compared to controls after 5 dpf calculated by area of confocal ventricular slice. Hc was defined as an oval indicated by red outline in (B). The area of the oval was calculated by the equations shown with lengths of a1, a2, b1, b2 measured by ImageJ in (B and C). (D-G) Ventricular HuC/D+ cells coexpress GABAergic lineage marker dlx5/6:GFP (Yu et al., 2011) in the 3 dpf Hc. Three confocal channel-split magnified images of the region depicted by the yellow rectangle in (D) are shown in (E-G). (H-J) Normal th2:GFP+ cells in the Hc of a 3 dpf lef1 mutant. (K-M) Increased BLBP+ cells in the Hc of a 3 dpf lef1 mutant. Higher magnification views of yellow rectangles in single channel are shown in the insets. (N-S) Normal proliferation in the Hc of a 5 dpf lef1 mutant as shown by pH3+ (N) and PCNA+ (O-Q) cells, and 1 day BrdU labeling (R and S). (T-V) BrdU pulse-chase (T) to measure birth of 5-HT+ and HuC/D+ cells. lef1 mutants have fewer 5-HT+/BrdU+ cells (U) and ventricular HuC/D+/BrdU+ cells (V). Data are mean ± SEM, except mean ± SD in (A, U, V). ***P < 0.001, **P < 0.01, *P < 0.05, ns. P > 0.05 by two-tailed unpaired Student's t tests. All images are confocal ventricular slices. All scale bars are 25 µm except 12.5 µm in (E) (see Table 3.1 for description of quantification and experimental n). |
