| OCR Text |
Show 12 zebrafish revealed robust adult neurogenesis in almost all the regions that are neurogenic during embryogenesis, including the hypothalamus (Grandel et al., 2006). Work from our lab has confirmed the generation of neurons expressing the panneuronal marker HuC/D, the GABA lineage marker Tg(dlx6a-1.4dlx5a-dlx6a:GFP) and GABA, serotonin (5-HT), and the dopaminergic marker Tg(th2:GFP-Aequorin)zd201 (th2:GFP) in the adult zebrafish hypothalamus (McPherson et al., 2015; Wang et al., 2012). However, BrdU-labeled newborn th2:GFP cells at 6 mpf are much fewer than those generated in the larval zebrafish hypothalamus (McPherson et al., 2015). Although it was claimed that more robust adult hypothalamic neurogenesis occurs in zebrafish than in mammals, no between-species comparisons throughout postembryonic and adult stages have been performed. It would be interesting to perform such studies in both zebrafish and mice with an optimized BrdU delivery method, and then compare the results between these two species. 1.4. Zebrafish adult neurogenesis outside the hypothalamus Since adult neurogenesis in zebrafish is observed in many CNS regions (Grandel et al., 2006), understanding its function in areas outside the hypothalamus may shed light on its function within the hypothalamus. Such regions include the telencephalon, cerebellum, optic tectum and hypothalamus (Adolf et al., 2006; Dirian et al., 2014; Grandel et al., 2006; Ito et al., 2010; Kaslin et al., 2009). One of the advantages in studying these three regions is the accessibility for imaging, especially live imaging. However, one has to consider that the adult zebrafish is not transparent, and therefore, is difficult to use for live imaging. If postembryonic neurogenesis in larva zebrafish can be shown to behave the same as, or |
