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Show COLLEGE OF HEALTH UNDERGRADUATE RESEARCH ABSTRACTS DOES GAMMA-TOCOPHEROL LOWER BLOOD PRESSURE IN DIABETIC MICE? Makenzie Hawkins, Paula Fernandes, Elisee Jionang Dapeau, Xin Wan, Elizabeth Johnson, Lance Deeter, Cyan Zhang, (Leena Panneerseelan, YouYou Li, Richard S. Bruno, John David Symons, Thunder Jalili) Division of Nutrition, Division of Endocrinology, Metabolism and Diabetes University of Utah and Department of Nutrition, Ohio State University Hypertension is a co-morbidity associated with diabetes and metabolic syndrome and nearly 75 million Americans have hypertension. G a m m a tocopherol (GTC), an isoform of Vitamin E, has protective effects on the endothelium of arteries; the endothelium is in part responsible for regulating arterial relaxation and reducing blood pressure (BP). In this study w e used diabetic mice (DB) with hypertension to determine if GTC can reduce blood pressure. Eight-week-old mice were divided into 3 separate groups: wild-type (WT) mice fed control (CON) diets (n=12), DB mice fed C O N diets (n=12), and DB mice fed GTC supplemented diets (0.1 %, n=12). BP was measured every week for 8 wk, 1 w k acclimation period. Body weights were measured every wk and morphometric values were measured at the end of the 8-wk period. There was no statistically significant difference in systolic and diastolic BP or heart rate between DB-GTC and DB-CON. Unexpectedly, BP and heart rate were higher (P<0.05) in WT-CON throughout the study. Even though both groups of DB mice started the study with similar body weights, after 8 weeks of GTC supplementation, DB-GTC mice had lower (P<0.05) final body weight versus the DB-CON group. In conclusion, a GTC supplemented diet did not reduce BP, however, GTC may have blunted weight gain and adipose tissue mass in DB-GTC compared to DB-CON. Future studies will determine if the reduction in body weight was due to reduced food consumption or a GTC specific effect on metabolism. Table 1 Initial Body wt, g Final Body wt, g Hxlwt,mg Liver wt, m g Adipose tissue, mg Fasting glucose, mg/dl Total Cholesterol HDL Triglycerides WILD-CON 23.0 ±1.1 24.1 ± 1.5 127 ± 13 557±U 735 ±21 89 ±23 108 ±22 39 ±12 92 ±58 DB-CON 40.1 ±4.1* 44.1 ± 3.6* 134 ±18 1,438 ±267* 2,693 ± 323* 525 ±112* 144 ±23 75 ±17* 145 ± 52* DB-GTC 40.16 ±2.4* 38.4 ± 5.5** 127 ±10 1,187 ±340* 2,424 ± 322* 511 ±34* 118.12 ±12 74.125 ±23* 135.25 ±42* Values are Mean ± SD, *P<0.05 vs. WT-CON, # P<0.05 vs. DB-CON Cyan Zhang Thunder Jalili John David Symons Makenzie Hawkins Paula Fernandes D'Elia Elisee Jionang Dapeau Elizabeth Johnson 37 |
