| OCR Text |
Show 2.8.1 Specific Growth Rate Specific growth rate between each consecutive time period was calculated using the logistic growth equation: for i = 1, where 2.8.2 ... , n Rj = = Nj = growth rate between time i and time j time i number of cells at time i for replicate j Cumulative Area Under the Curve Cumulative area under the curve (increase in number of cells) from hour 0 through the final time . period (n) was calculated as: " Aj = tl *(NiJ - No)/2 + L e, T,,) - i-I * (N,,-IJ + NIIj - I 2*No)/2 / where = area under the t, = time i Njj = growth curve for replicate j number of cells at time i for replicate j The statistical computer package TOXIS® (EcoAnalysis, INC. 1994) was used to estimate the EC50 value and the 95% confidence interval based on results from specific growth rate and cumulative area under the growth curve calculations. Parametrix, Inc. Final Report 7 March 1998' |
