| OCR Text |
Show Table 1. Summary of test conditions for the chronic Dunaliella viridis toxicity test. Test Title: Evaluation of Selenium Toxicity to the Algae, Dunaliella viridis Test Material: Sodium selenate (CAS #13410-01-0) Test Organisms/Age: Dunaliella viridis; 4 to 7 days from culture renewal Source: In-house culture, originally obtained from Murdoch University School of Biological and Environmental Sciences NumberITest Chamber:' 10,000 cells/mL at test initiation VolumelTest Chamber: 50 mL Test Concentrations: 11, 18, 30, 49, 84, and 135 mgIL selenium, measured. Replicates: Sixteen (four terminated per day/concentration) Test Duration: 96 hours Control/Dilution Media: 1 urn filtered GSL water Test Chambers: 125 mL glass Erlenmeyer flasks Lighting: Fluorescent bulbs (400 ± 40 foot candles) Photoperiod: Continuous illumination Shaking Rate: 100 rpm continuous Temperature: 25 ± 1°C Chemical Data: Temperature at test initiation and every 24 hours; salinity and pH at test initiation and termination in the high, medium and low concentrations and the control; samples for selenium analysis collected at test initiation and termination. Effect Measured: Cell growth Test Acceptability: Control 2.0 x lOS cells/mL; <20% coefficient of variation in cell density among controls Parametrix, Inc. Final Report 5 March 1998 |
