OCR Text |
Show 1906.] PLACENTA IN UNGULATES. 89 alcohol and ammonia) 50 m m . deep was examined in a long spectroscope bottle, but no bands except the following could be seen :- (1) X 594-5 - 572-5, (2) X 556-5- 531. " Other experiments were made to prove that the pigment was not hsematin ; e. g. ammonium sulphide was added, but no reduced haeniatin spectrum appeared. Caustic alkalis also failed to change the spectrum into that of alkaline haematin, acids into that of acid haematin, which would have occurred if that pigment had been present. u Let us now compare the spectrum of the pigment in alcoholic solution with that of an as equally as possible dilute aqueous solution of oxyhaemoglobin :- Placenta pigment. Oxyhemoglobin. (1) X 593 - 574, (1) X 586 - 568, (2) X 556-5 - 531, (2) X 552 - 525. " (See Hoppe-Seyler, Handbuch, 1903.) 11 This pigment therefore appears to be more nearly related to haematoporphyrin than to any other known decomposition-product of haemoglobin." IY. Solution obtained from the foetal cotyledons of the Cow by the second method. " The alcoholic solution shows the two-banded spectrum, is the colour of fairly deep sherry; filtered, the filtrate is reddish yellow and shows two bands :- (1) X 593 - 577, second reading X 594'5 - 575, (2) X 556-5 - 534-5, second reading X 556*5 - 533*5, and in addition a faint shading nearer the violet. " The solution was evaporated on the water-bath, but owing to the presence of some fatty matter remained fluid while hot. " A n aqueous solution of the residue is faintly yellow, but shows no bands or fluorescence. " The residue was brownish, in thin parts brownish yellow. The absolute alcohol solution of this residue is reddish yellow with a suspicion of greenish fluorescence; it gives two faint bands :- (1) X 593-574, (2) X 558 - 536. " The violet end of the spectrum is cut off at \ 487. " Ammonia produces turbidity, and diminishes, in the filtrate, the intensity of the bands, but their position is unaltered. " O n adding a little H.,S04 to an alcoholic solution slight precipitation is produced. The filtrate is of a deep yellow^ sherry-colour and the bands referred to above (in the Sheep) resembling |