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Show Molecular and Functional Characterization of SCAM Pin t\w Drosophila Nervous System T. \\n\ kn, M. Sinj th. K. Gnu tl Ic* department nf H i «lfi £y, I. im t^r* it v rtf1: Jab • • •.! "^ Dtp n rt im ii t of Hi olo^y, Va n JtTbilt U iii vcj'sitv ... ;•„;;. nudLL-suw-BASC' «l3n«y.:d I'ii- ^-5" .I, I -.-I II-.- ! I'-: ... ;-nl i.|x^ :\;-i |....^..U-:I 1=.. X.,.:, ....... .... .„ • ,|... , |..x *<:AMf:-jii;nfi--iiii: wtTl fco irrft-np-d info Ihc- -Ri1 gm n. IV I--I: ^:.n:.pi . •i'- .-ii : ii- ;••..•.!.'•--hi h-tf-J f:'i i.: I Till tj -,- ^.i^ L 1:1 ., |x I ••.:, '•tfGti'f lUllllOl W K '• '-ll1 ..ill.-!- -II. Vesicle recycling is a critical aspect of fast and maintained synaptic transmission in the nervous system, A relatively uncharactenzed class of synaptic vesicle (SV) integral proteins, Secretory Carrier Associated Membrane Proteins (SCAMP) may play a central role in nucleating clathrin mediated endocytosis at nerve terminals. Preliminary biochemical assays of mammalian SCAMPs suggest they link, the membrane to the cylosolic endocytosis maclnneiy (Fetnandez CbacoiK Achiriloaie et al, 2000), We propose to bring the tools of the Drosophila genetic system to bear on this question. The Drosophila nervous system offers a powerful model to assess protein localization, genet. 50 |
