Journal of Neuro-Ophthalmology, March 2006, Volume 26, Issue 1

Relationship Beween Cognitive Impairment and Retinal Morphological and Visual Functional Abnormalities in Alzheimer Disease

BACKGROUND: There is conflicting evidence as to whether Alzheimer disease (AD) is accompanied by loss of retinal ganglion cells. To evaluate this issue, we have used optical coherence tomography (OCT) to assess the thickness and volume of the retina. We have also sought to correlate our findings with visual function and cognitive impairment. METHODS: We evaluated 28 eyes of 14 patients with AD and 30 eyes of 15 age-matched control subjects. In these two groups, we measured retinal nerve fiber layer (RNFL) thickness, macular thickness, and macular volume with OCT, visual function through latency of the pattern visual evoked potential (VEP) signal, and cognitive impairment through the Mini-Mental State Examination (MMSE). RESULTS: The parapapillary and macular RNFL thickness in all quadrants and positions of AD patients were thinner than in control subjects. The mean total macular volume of AD patients was significantly reduced as compared with control subjects (P < 0.05). Total macular volume and MMSE scores were significantly correlated. No significant difference was found in the latency of the VEP P100 of AD patients and control subjects. CONCLUSIONS: Our study confirms some other studies in showing that in AD patients there is a reduction of parapapillary and macular RNFL thickness and macular volume as measured by OCT. The reduction in macular volume was related to the severity of cognitive impairment.

ORIGINAL CONTRIBUTION Relationship between Cognitive Impairment and Retinal Morphological and Visual Functional Abnormalities in Alzheimer Disease Pervin K. Iseri, MD, Ozgiil Altina § , MD, Tomris Tokay, MD, and Nur § en Yiiksel, MD Background: There is conflicting evidence as to whether Alzheimer disease ( AD) is accompanied by loss of retinal ganglion cells. To evaluate this issue, we have used optical coherence tomography ( OCT) to assess the thickness and volume of the retina. We have also sought to correlate our findings with visual function and cognitive impairment. Methods: We evaluated 28 eyes of 14 patients with AD and 30 eyes of 15 age- matched control subjects. In these two groups, we measured retinal nerve fiber layer ( RNFL) thickness, macular thickness, and macular volume with OCT, visual function through latency of the pattern visual evoked potential ( VEP) signal, and cognitive impairment through the Mini- Mental State Examination ( MMSE). Results: The parapapillary and macular RNFL thickness in all quadrants and positions of AD patients were thinner than in control subjects. The mean total macular volume of AD patients was significantly reduced as compared with control subjects ( P < 0.05). Total macular volume and MMSE scores were significantly correlated. No significant difference was found in the latency of the VEP P100 of AD patients and control subjects. Conclusions: Our study confirms some other studies in showing that in AD patients there is a reduction of para­papillary and macular RNFL thickness and macular volume as measured by OCT. The reduction in macular volume was related to the severity of cognitive impairment. { J Neuro- Ophthalmol 2006; 26: 18- 24) A lzheimer disease ( AD) is the most common degener­ative dementia and causes a progressive decline in cog­nitive function. In most cases, an episodic memory deficit is the predominant initial complaint. As time passes, changes Departments of Neurology ( PKI, TT) and Opthalmology ( OA, NY), Kocaeli University Medical School, Kocaeli, Turkey Address correspondence to Pervin K. Iseri, MD, Kocaeli University, Department of Neurology, Derince Kocaeli 41100 Turkey; E- mail: pervin. iseri@ gmail. com in daily living activities, cognitive functions, and visual disturbances supervene. Visual disturbances consist of impairment in spatial contrast sensitivity ( 1), motion perception ( 2,3), color dis­crimination ( 4), and blurred vision ( 5). Several recent arti­cles have attributed the visual dysfunction in AD to damage in primary visual cortex and to degeneration of higher cortical areas ( 6- 8). Other studies have shown evidence of pre- cortical involvement on the basis of reduction in the number of retinal ganglion cells and axons of the optic nerve ( 9,10). In contrast to these reports, some histopath­ologic studies have shown no retinal nerve damage in AD ( 11,12). Although recent studies have used sophisticated imaging techniques, such as optical coherence tomography ( OCT), scanning laser polarimetry, and pattern electroret-inography ( PERG), to assess the morphologic and func­tional changes of the retina in AD, disagreement about retinal involvement persists ( 13- 15). The macula is denned anatomically as that region of the retina where the ganglion cell layer has a thickness of more than one cell. The ganglion cells and retinal nerve fiber layer ( RNFL) contribute 30% to 35% of retinal thick­ness in the macula, where the ganglion cells are known to be most concentrated ( 16). Blanks et al ( 17) histo­logically observed a total decrease of 25% of neurons in the ganglion cell layer at the level of the fovea/ parafoveal retina in AD. The greatest decrease was in the foveal region. To our knowledge, no previous studies have investigated macular thickness and volume in living AD patients. OCT is a relatively new non- invasive, non- contact, transpupillary imaging technology that provides high-resolution cross- sectional images of the retina. OCT has been reported to be useful in assessing glaucoma, diabetic neuropathy, and macular edema ( 18- 22). The aim of this study was to investigate whether a correlation exists between structural ( RNFL thickness, macular thickness and volume) and functional ( visual evoked potential [ VEP]) measures and cognitive impair­ment in AD. 18 J Neuro- Ophthalmol, Vol. 26, No. 1, 2006 Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited. Alzheimer Disease J Neuro- Ophthalmol, Vol. 26, No. 1, 2006 METHODS Patient Accrual We compared 28 eyes of 14 patients with AD to 30 eyes from 15 age- matched control subjects. The AD patients were obtained from the Kocaeli University Neu­rology Department Dementia Clinic; the control subjects were obtained from the Kocaeli University Eye Diseases Department General Clinic, Kocaeli, Turkey. The patients met criteria for probable AD set by the National Institute of Neurological and Communicative Disorders and Stroke- Alzheimer's Disease and Related Disorders Association ( NINCDS- ADRDA) ( 23) and DSM- IV AD patients had mild and moderate cognitive impairment according to the Clinical Dementia Rating ( CDR) scale ( 24). All partic­ipants were free of ocular disease and systemic disorders affecting vision. Informed consent of AD patients was ob­tained from their first- degree relatives. The research followed the tenets of the Declaration of Helsinki, and the protocol was approved by the local ethics committee. Neurological Assessments Each AD patient underwent a detailed neurological examination including laboratory, neuro- imaging evalua­tions, and psychometric testing. The control subjects also underwent a detailed neurological examination to rule out the presence of cognitive impairment. We excluded patients with dysmetabolic diseases, a history of alcohol abuse, psy­chiatric disorders, or other neurological diseases. Mini- Mental State Examination ( MMSE) ( 25), Blessed short orientation, and Clock drawing tests for clinical evaluation were used to assess cognition in AD and control subjects. Ophthalmological Assessments All AD patients and control subjects underwent a complete ophthalmologic examination, including assess­ment of visual acuity, refraction, ocular motility, pupillary reflexes, anterior and posterior segment biomicroscopy, applanation tonometry, dilated fundus examination, and Octopus 101 perimeter program G2 visual field testing. The visual field results were uniformly normal in AD and con­trol subjects. All participants had a corrected visual acuity of 5/ 10 or better with a refractive error between ± 3 spheric diopters and intraocular pressures less than 22 mmHg. Eyes with posterior pole pathology such as macular degenera­tion, glaucoma suspect, or glaucoma, or patients with media opacification such as cataract that prevented ocular and OCT examination were excluded. Visual Evoked Potential Examination All AD patients and control subjects underwent VEP ex­amination. The VEP was generated using a black- and- white checkerboard pattern on a television monitor with a di­mension of 5 X 5 cm for every check under the following conditions: contrast 95%, check size subtense 50', reversal rate 2 sec- 1, mean luminance 12 Cd. rrT2 field size 17° X 14°. One hundred stimulus epochs of 200 msn were obtained in each average and the amplifier bandwidth was 1- 100 Hz. Patients sat one meter away from the monitor. Ag/ AgCl cup- shaped electrodes fixed with collodion were placed over the left and right occiputs at 01 and 02 with a com­mon reference at Fpz and a ground on the left arm. The patients' gaze was fixed on a point at the center of the television monitor monocularly The bioelectric signal was filtered ( bandpass 0.5- 200 Hz). Two hundred responses were averaged for every trial ( Neuropack Nihon Kohden, MEB- 5504 K, Tokyo, Japan). The analysis time was 250 milli­seconds. Sweep length was 300 milliseconds ( 30 msec/ div) and stimulus rate was 1 Hz. The normal range of P100 peak latency is 100.26 ± 7.04 milliseconds based on testing of normal controls in our laboratory. Peak latencies of the PI00 component were measured. Optical Coherence Tomography Examination All AD patients and control subjects underwent OCT examination. The RNFL thickness, and macular thickness and volume were measured by OCT Model 3000 unit ( Model 3000, software version Al. l, Carl Zeiss Meditec, Inc., Dublin, California, USA) after pupillary dilatation. Tomography images were constructed from a series of axial reflectance profiles ( A- scans) over 2 mm of depth in less than 1 second. Retinal thickness and RNFL thickness were calculated by processing the cross- sectional images using computer algorithms to detect boundaries by searching each A- scan for the highest rates of changes in reflectivity. The software allows the mapping of the thickness data ac­cording to quadrant- by- quadrant and clock- hour analyses. Retinal thickness was determined by computer as the distance between the first reflection at the vitreo- retinal interface and the anterior boundary of the second reflective layer, corresponding to the retinal pigment epithelium and the choriocapillaris. RNFL thickness was automatically assessed by computer assuming the correlation with the red highly reflective layer at the vitreo- retinal interface. The pos­terior margin of the RNFL was automatically located by starting within the photoreceptor layer ( posterior) and search­ing forward in the image ( 19,26,27). Throughout scanning, the patient kept both eyes con­stantly fixed on an internal target provided by the equip­ment. Each subject eye underwent fast RNFL and macula scan protocols. Mean OCT values were calculated from the values of the three scans. One of the authors ( OA) performed the image acquisition and judged its quality. Scans with poor image quality were defined as scans with signal- to- noise 19 Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited. J Neuro- Ophthalmol, Vol. 26, No. 1, 2006 Iseri et al ratio of less than 45dB or excessive eye movement during measurement. We excluded three patients who had difficulty in cooperating with testing. The fast RNFL scan protocol consisted of three consecutive 360° circular scans with a diameter of 3.4 mm centered on the optic disc, each containing 256 A- scans taken in a single session of 1.92 seconds. The parapapillary RNFL thickness parameters evaluated in this study were average thickness ( 360° measurement), temporal quadrant thickness ( 316° to 45°), superior quadrant thickness ( 46° to 135°), nasal quadrant thickness ( 136° to 225°), inferior quad­rant thickness ( 226° to 315°), and thickness for each 12: 00 position with the 3: 00 as nasal, 6: 00 position as inferior, 9: 00 as temporal, and 12: 00 position as superior. The fast macula scan protocol consisted of six consecutive 6 mm radial line scans centered on the macula, each containing 128 A- scans taken in a single session of 1.92 seconds. Six sets of intersecting and equally spaced scans were obtained, each crossing the central fovea. The retinal thickness/ volume tubular analysis program was used to evaluate macular scans. This analysis program presents mean foveal thickness and total macular volume in 3.5 and 6.00 mm macular maps. Macular retinal thickness data were displayed in three concentric circles. The central disk was the foveal region measuring 1.00 mm in diameter. The inner and outer rings were each divided into four quadrants; the rings had diameters of 3 mm and 6 mm, respectively, in 6.00 mm macular maps. An average retinal thickness and volume were reported for each of the nine regions. Statistical Analysis The data are reported as mean values ± standard deviation ( SD). The differences between AD and control eyes were statistically evaluated with the Student t test. To assess whether a correlation existed between OCT, VEP, and clinical severity of disease, Pearson's correlation test was used. P < 0.05 was considered significant. TABLE 1. Demographic and clinical data of groups AD Control P value Age 70.1 ± 9.7 65.1 ± 9.8 0.180 ( mean ± SD) ( 52- 83) ( 49- 79) Gender 0.837 Men 6 7 Women 8 8 MMSE 18.5 ± 6.3 29.4 ± 0.6 0.000* ( mean ± SD) ( 8- 28) ( 29- 30) VEP P100 107.96 ± 9.93 107.80 ± 10.40 0.950 Amplitude 16.35 ± 2.69 18 ± 3.59 0.053 ( 12- 23) ( 14- 24) * P < 0.01. AD, Alzheimer disease; MMSE, Mini- Mental State Examination; SD, standard deviation; VEP, visual evoked potential. compared with control subjects ( 113.16 ± 6.72 | jim) ( P < 0.05) ( Table 2). The RNFL thickness in all quadrants and positions of AD patients was thinner than in control sub­jects. RNFL differences were statistically significant ( P < 0.05) except in the temporal quadrant, 8: 00 and 9: 00 posi­tions ( Table 1). The retinal thickness in all quadrants of the macula of AD patients was less than in control subjects. This thinning was prominent in the nasal, temporal, and inferior quad­rants ( P < 0.05) ( Table 2). The macular volume of the AD patients was less than that of control subjects in all macular regions except the foveal minimum. The reduction in mac­ular volume was statistically significant except in the nasal and inferior inner quadrants and the temporal and superior outer quadrants of the macula. The mean total macular vol­ume of AD patients ( 6.80 ± 0.41 mm3) was significantly re­duced when compared with that of control subjects ( 7.10 ± 0.23 mm3) ( P < 0.05) ( Table 1). RESULTS The mean ages of AD and control groups were 70.1 ± 9.7 years and 65.1 ± 9.8 years, respectively. There was no statistically significant difference in age and gender be­tween groups. Demographic and clinical data of AD and control groups are shown in Table 1. Examples of OCT and VEP recordings from control and AD eyes are shown in Figure 1. Optical Coherence Tomography The mean RNFL average thickness was significantly reduced in AD patients ( 87.46 ± 23.78 microns) when Visual Evoked Potentials No significant difference was found in the latencies and amplitudes of the VEP P100 of AD patients ( 107.96 ± 9.93 msec) ( 16.35 ± 2.69 | JLV) and control subjects ( 107.80 ± 10.40 msec) ( 18 ± 3.59 | JLV) ( P > 0.05) ( Table 1). Correlation between Optical Coherence Tomography, Visual Evoked Potential, and Mini- Mental State Examination A highly significant correlation was found between total macular volume and MMSE scores in AD patients ( r = 0.696; P = 0.006) ( Fig. 2). There was no correlation between OCT and VEP changes ( Table 3). 20 © 2006 Lippincott Williams & Wilkins Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited. Alzheimer Disease J Neuro- Ophthalmol, Vol. 26, No. 1, 2006 0 100 200 300 100 500 pn FIG. 1. Exampleof retinal nerve fiber layer ( RNFL) thickness in one eye of a patient with Alzheimer disease ( AD) ( A) and one eye of a control subject ( B). Top: Circular optical coherence tomography ( OCT) taken in cylindri­cal section of tissue surrounding the optic disc shows a marked decrease of the RNFL reflection in the eye of the AD patient ( A) as compared with the eye of a control subject ( B). Bottom: The RNFL thickness in each clock position and the macular thick­ness in each region are reduced in the AD eye ( A) as compared with the control subject eye ( B). DISCUSSION The results of our study suggest a significant reduction in parapapillary RNFL, macular thickness and volume in patients with AD. We have also demonstrated a highly significant correlation between total macular volume and MMSE scores. Structural changes were not significantly correlated to the VEP changes. Our data are consistent with histologic studies ( 9,10) and other methods of evaluating the RNFL and optic nerve in vivo ( 15,28), which demonstrate substantial decline in the quantity of optic nerve fibers and a degeneration of ret­inal ganglion cells in AD. Optic disc pallor, pathologic disc cupping, and thinning of the neuroretinal rim and the RNFL have been reported in two clinical studies based on the sub­jective evaluation of fundus photographs ( 28,29). Tsai et al ( 28) have observed an increased cup- to- disc ratio, cup volume, and decreased disc rim area in AD patients by optic nerve analyzer. Parisi et al ( 15) demonstrated a reduction in RNFL thickness by using OCT and suggested that this morphologic abnormality is related to retinal dysfunction as revealed by abnormal PERG responses. Our data indicate not only a significant decline in parapapillary RNFL but also in macular thickness and volume in AD eyes. These findings suggest a loss of retinal ganglion cells in AD patients. To our knowledge, no report has previously documented the macular volume and thick­ness in vivo in AD patients. These findings are in agreement with the postmortem study of Blanks et al ( 17) demon­strating a total decrease of 25% of neurons in the ganglion cell layer at the level of the foveal and parafoveal retina. In the pathophysiology of AD, beta- amyloid peptides that are cleaved from the amyloid precursor protein ( APP) play a critical role. In the later stages of the disease, FIG. 6 . 0 6.5 7.0 Total Macular Volume ( cubic mm) 2. Correlation plot of the total macular volume and Mini- Mental State Exam ( MMSE) scores in our Alzheimer disease ( AD) patients shows a high correlation ( r = 0.696). 21 Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited. J Neuro- Ophthalmol, Vol. 26, No. 1, 2006 Iseri et al TABLE 2. Optical coherence tomography parameters of subjects Parameter Location Alzheimer group ( n = 28), mean ± SD Control group ( n = 30), mean ± SD Parapapillary RNFL thickness ( microns) Average foveal/ macular thickness ( microns) Foveal/ macular volume ( cubic mm) RNFL ( average) Superior RNFL Inferior RNFL Temporal RNFL Nasal RNFL 1: 00 2: 00 3: 00 4: 00 5: 00 6: 00 7: 00 8: 00 9: 00 10: 00 Thickness at 11: 00 12: 00 Foveal minimum Fovea Temporal inner macula Superior inner macula Nasal inner macula Inferior inner macula Temporal outer macula Superior outer macula Nasal outer macula Inferior outer macula Fovea Temporal inner macula Superior inner macula Nasal inner macula Inferior inner macula Temporal outer macula Superior outer macula Nasal outer macula Inferior outer macula Foveal minimum 87.46 112.64 103.10 64.92 63.57 103.53 81.00 49.96 60.39 87.17 111.96 111.17 67.82 51.03 76.89 117.14 118.35 194.57 200.46 257.57 269.60 265.46 264.78 224.57 245.50 245.25 227.78 0.153 0.400 0.418 0.413 0.410 1.197 1.297 1.296 1.183 6.809 •+• •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- 23.78 35.32 33.64 17.70 19.09 40.12 27.87 12.78 22.59 26.65 38.01 44.86 21.69 13.11 23.95 36.60 40.53 30.54 20.74 21.14 23.23 26.61 34.54 17.60 13.01 21.82 23.07 0.01 0.03 0.03 0.04 0.05 0.11 0.06 0.11 0.10 0.41 113.16 137.16 141.56 72.30 96.00 131.43 114.13 79.83 95.33 129.83 153.83 142.06 74.03 53.83 90.16 138.80 142.03 165.26 218.25 267.96 279.13 277.23 280.16 233.43 247.70 264.93 241.36 0.160 0.420 0.432 0.430 0.435 1.224 1.315 1.394 1.267 7.106 •+• •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- •+- 6.72 16.48 19.09 16.42 34.39 24.22 39.26 39.20 28.69 31.85 24.71 26.35 19.11 12.33 22.37 27.23 27.17 22.12 24.68 19.35 12.05 14.51 11.78 14.17 9.33 12.24 10.30 0.01 0.01 0.01 0.02 0.01 0.06 0.04 0.07 0.06 0.23 0.000 0.002 0.000 0.106 0.000 0.003 0.001 0.000 0.000 0.000 0.000 0.002 0.251 0.406 0.033 0.013 0.013 0.000 0.004 0.056 0.060 0.045 0.032 0.039 0.460 0.000 0.007 0.005 0.006 0.081 0.068 0.027 0.272 0.264 0.000 0.000 0.002 RNFL, retinal nerve fiber layer; SD, standard deviation. beta- amyloid peptides compose the characteristic pathologic findings of AD, including neurofibrillary tangles and neuritic plaques. The cortical degeneration characteristic of AD is present especially in visual association areas. Histopathologic studies have disclosed the pathologic hallmarks of AD ( B- amyloid, tau, and APP neurofibrillary tangles and neuritic plaques) in subcortical visual centers, including the lateral geniculate nucleus and superior colliculus ( 31) but not in the retina ( 9,30). On the other hand, degeneration of retinal ganglion cells and their axons in the nerve fiber layer has been reported ( 10). Morpho-metric analysis has shown that in AD, the optic nerve has 22 © 2006 Lippincott Williams & Wilkins Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited. Alzheimer Disease J Neuro- Ophthalmol, Vol. 26, No. 1, 2006 TABLE 3. Correlations between visual evoked potential PlOO latency, Mini- Mental State Examination score, and optic coherence tomography retinal thickness measures and optic coherence tomography parameters in Alzheimer disease Versus RNFL average thickness Foveal thickness Total macular volume VEP PlOO latency Correlation coefficient ( r) 0.318 - 0.096 0.189 P 0.099 0.627 0.335 Mini- Mental State Examination score Correlation coefficient ( r) 0.071 0.004 0.696 P 0.810 0.989 0.006 RNFL, retinal nerve fiber layer; VEP, visual evoked potential. predominant loss of the largest class of retinal ganglion cells ( M- cells) ( 10). The loss of retinal ganglion cells may be a primary process or a consequence of retrograde neurodegeration occurring in the cortical regions. We found no abnormalities in VEP in our AD pa­tients. Normal pattern VEP responses have been described before in AD, although there is evidence that the P2 com­ponent of pattern VEP is delayed ( 32,3 3). In an earlier report, progressive increase in the latency of the flash VEP was related to the severity of dementia ( 33). These findings stem largely from the fact that flash VEP reflects the abnormality of visual association regions of the brain while pattern VEP shows the function of primary visual cortex and visual pathways ( 34). Sparing of the primary visual cortex with extensive cortical disease has been shown in AD ( 33) with positron emission tomography and histopathologic studies ( 35,36). The normal VEP responses in our study suggest that primary cortical region and optic nerve function is normal despite considerable RNFL loss. The significant correlation we found between MMSE scores and macular volume as measured by OCT may be useful in providing a basis for further studies evaluating the effect of disease severity on RNFL. REFERENCES 1. Hutton JT, Morris JL, Elias JW, Poston JN. Contrast sensitivity dysfunction in Alzheimer's disease. Neurology 1993; 43: 2328- 30. 2. Gilmore GC, Wenk HE, Naylor LA, Koss E. Motion perception and Alzheimer's disease. J Gerontol 1994; 49: P52- P57. 3. Trick GL, Silverman SE. Visual sensitivity to motion: age related changes and deficits in senile dementia of the Alzheimer's type. Neurology 1991; 41: 1437^ K). 4. Cronin- Golomb A. Vision in Alzheimer's disease. Gerontologist 1995; 35: 370- 76. 5. Cronin- Golomb A, Corkin S, Rizzo JF, Cohen J, Growdon JH, Banks KS. Visual dysfunction in Alzheimer's disease: relation to normal aging. Ann Neurol 1991; 29: 41- 52. 6. Amstrong RA. Visual field defects in Alzheimer's disease patients may reflect differential pathology in the primary visual cortex. Optom Vis Sci 1996; 73: 677- 82. 7. Morrison JH, Hof PR, Bouras C. An anatomic substrate for visual disconnection in Alzheimer's disease. Ann NY Acad Sci 1991; 640: 36^ 3. 8. Mountjoy CQ, Roth M, Evans NJ, Evans HM. Cortical neuronal counts in normal elderly controls and demented patients. Neurobiol Aging 1983; 4: 1- 11. 9. Hinton DR, Sadun AA, Blanks JC, Miller CA. Optic nerve degeneration in Alzheimer's disease. N EnglJ Med 1986; 315: 485- 7. 10. Sadun AA, Bassi CJ. Optic nerve damage in Alzheimer's disease. Ophthalmology 1990; 97: 9- 17. 11. Curcio CA, Drucker DN. Retinal ganglion cells in Alzheimer's disease and aging. Ann Neurol 1993; 33: 248- 57. 12. Davies DC, McCoubrie P, McDonald B, Jobst KA. Myelinated axon number in the optic nerve is unaffected by Alzheimer's disease. Br J Ophthalmol 1995; 79: 596- 600. 13. Kergoat H, Kergoat MJ, Justino L, Chertkow H, Robillard A, Bergman H. An evaluation of the retinal nerve fiber layer thickness by scanning laser polarimetry in individuals with dementia of the Alzheimer type. Acta Ophthalmol Scand 2001; 79: 187- 91. 14. Kergoat H, Kergoat MJ, Justino L, Robillard A, Bergman H, Chertkow H. Normal optic nerve head topography in the early stages of dementia of the Alzheimer type. Dement Geriatr Cogn Disord 2001; 12: 359- 63. 15. Parisi V, Restuccia R, Fattapposta F, Mina C, Bucci MG, Pierelli F. Morphological and functional retinal impairment in Alzheimer's disease patients. Clin Neurophysiol 2001; 112: 1860- 7. 16. Zeimer R, Asrani S, Zou S. Quantitative detection of glaucomatous damage at the posterior pole by retinal thickness mapping. A pilot study. Ophthalmology 1998; 105: 224- 31. 17. Blanks JC, Torigoe Y, Hinton DR, Blanks RH. Retinal pathology in Alzheimer's disease. I. Ganglion cell loss in foveal/ parafoveal retina. Neurobiol Aging 1996; 17: 377- 84. 18. Guedes V Schuman JS, Hertzmark E, Wollstein G, Correnti A, Mancini R, et al. Optical coherence tomography measurement of macular and nerve fiber layer thickness in normal and glaucomatous human eyes. Ophthalmology 2003; 110: 177- 89. 19. Hee MR, Izatt JA, Swanson EA, Huang D, Schuman JS, Lin CP, et al. Optical coherence tomography of the human retina. Arch Ophthalmol 1995; 113: 325- 32. 20. Hee MR, Puliafito CA, Wong C, Duker JS, Reichel E, Rutledge B, et al. Quantitative assessment of macular edema with optical coherence tomography. Arch Ophthalmol 1995; 113: 1019- 2. 21. Hee MR, Puliafito CA, Wong C, Duker JS, Reichel E, Schuman JS, et al. Optical coherence tomography of macular holes. Ophthalmol­ogy 1995; 102: 748- 56. 22. Krivoy D, Gentile R, Liebmann JM, Stegman Z, Rosen R, Walsh JB, et al. Imaging congenital optic disc pits and associated maculopathy using optical coherence tomography. Arch Ophthalmol 1996; 114: 165- 70. 23. McKhann G, Drachman D, Folstein M, Katzman R, Price D, Stadlan EM. Clinical diagnosis of Alzheimer's disease: report of the NINCDS-ADRDAWork Group under the auspices of the Department of Health and Human Services Task Force on Alzheimer's disease. Neurology 1984; 34; 939^ 4. 24. Morris JC. The Clinical Dementia Rating ( CDR): current version and scoring rules. Neurology 1993; 43: 2412- 13. 23 Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited. J Neuro- Ophthalmol, Vol. 26, No. 1, 2006 Iseri et al 25. Folstein MF, Folstein SE, McHugh PR. " Mini- mental state". A practical method for grading the cognitive state of patients for the clinician. JPsychiatr Res 1975; 12: 189- 98. 26. Huang D, Swanson EA, Lin CP, Schuman JS, Stinson WG, Chang W, et al. Optical coherence tomography. Science 1991; 254: 1178- 81. 27. Schuman JS. Optical coherence tomography for imaging and quan­tification of nerve fiber layer thickness. In: Schuman JS. Imaging in Glaucoma. Thorofare, NJ: Slack Inc; 1997: 95- 130. 28. Tsai CS, Ritch R, Schwartz B, Lee SS, et al. Optic nerve head and nerve fiber layer in Alzheimer's disease. Arch Ophthalmol 1991; 109: 199- 204. 29. Hedges TR, Perez Galves R, Speigelman D, Barbas NR, Peli E, Yardley CJ. Retinal nerve fiber layer abnormalities in Alzheimer's disease. Acta Ophthalmol Scan 1996; 74: 271- 5. 30. Loffler KU, Edwards DP, Tso MO. Immunoreactivity against tau, amyloid precursor protein, and beta- amyloid in the human retina. Invest Ophthalmol Vis Sci 1995; 36: 24- 31. 31. Leuba G, Saini K. Pathology of subcortical visual centres in relation to cortical degeneration in Alzheimer's disease. Neuropathol Appl Neurobiol 1995; 21: 410- 22. 32. Harding GF, Wright CE, Orwin A. Primary presenile dementia: the use of the visual evoked potential as a diagnostic indicator. Br J Psychiatry 1985; 147: 532- 9. 33. Orwin A, Wright CE, Harding GF, Rowan DC, Rolfe EB. Serial visual evoked potential recordings in Alzheimer's disease. BMJ1986; 293: 9- 10. 34. Moore NC. Visual evoked responses in Alzheimer's disease: a review. Clin Electroencephalogr 1997; 28: 137^ 42. 35. Benson DF The use of positron emission scanning techniques in the diagnosis of Alzheimer's disease. In: Corkin S, David KL, Growdon JH, Usdin E, Wurtman RJ, eds. Alzheimer's Disease: A Report of Progress in Research. New York: Raven Press; 1982: 79- 82. 36. Brun A, Gustafson L. Distribution of the cerebral degeneration in Alzheimer's disease. A clinico- pathological study. Arch Psychiatr Nervenkr 1976; 223: 15- 33. 24 © 2006 Lippincott Williams & Wilkins Copyright © Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited.