Phosphorylation of cardiac troponin by guanosine 3':5'-monophosphate-dependent protein kinase.

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Publication Type Journal Article
School or College College of Pharmacy; School of Medicine
Department Biomedical Informatics; Biochemistry; Pharmacology & Toxicology
Creator Blumenthal, Donald K.
Other Author Stull, James T.; Gill, Gordon N.
Title Phosphorylation of cardiac troponin by guanosine 3':5'-monophosphate-dependent protein kinase.
Date 1978-01-25
Description Homogeneous cGMP-dependent protein kinase catalyzes the rapid incorporation of phosphate, specifically into the inhibitory subunit of purified cardiac troponin with a maximal incorporation of 1 mol of phosphate/mol of troponin. When troponin was incubated in the presence of both cGMP- and cAMP-dependent protein kinases, a maximal incorporation of 1 mol of phosphate/mol of troponin was observed which suggested phosphorylation of the same site by the two kinases. Both cyclic nucleotide-dependent kinases had similar Km values for troponin, but the Vmax value for the phosphorylation reaction catalyzed by cAMP-dependent protein kinase was 12-fold greater than the value obtained for cGMP-dependent protein kinase.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 253
Issue 2
First Page 324
Last Page 326
Subject Protein Kinases
Subject MESH Muscle Proteins; Pharmacology; Enzyme Activation; Myocardium; Kinetics; Troponin; Metabolism
Language eng
Bibliographic Citation Blumenthal DK, Stull JT,Gill GN. Phosphorylation of cardiac troponin by guanosine 3':5'-monophosphate-dependent protein kinase. J Biol Chem. 1978 Jan 25;253(2):324-6. Retrieved August 30,2006 from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=201626&query_hl=2&itool=pubmed_DocSum
Rights Management Copyright © American Society for Biochemistry and Molecular Biology, J Biol Chem., 253, 334-336, 1978
Format Medium application/pdf
Identifier ir-main,359
ARK ark:/87278/s6st87hk
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707357
Reference URL https://collections.lib.utah.edu/ark:/87278/s6st87hk
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