You've searched: Department: "Pharmacology & Toxicology" Collection: "ir_uspace"
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1 Blumenthal, Donald K.In vivo evaluation of the delivery and efficacy of a sirolimus-laden polymer gel for inhibition of hyperplasia in a porcine model of arteriovenous hemodialysis graft stenosisSynthetic arteriovenous (AV) hemodialysis grafts are plagued by hyperplasia resulting in occlusion and graft failure yet there are no clinically available preventative treatments. Here the delivery and degradation of a sirolimus-laden polymer gel were monitored in vivo by magnetic resonance imaging ...2012-01-01
2 Bernstein, Paul S.HPLC measurement of ocular carotenoid levels in human donor eyes in the lutein supplementation era.PURPOSE: A substantial proportion of the population at risk for visual loss from age-related macular degeneration consumes supplements containing high doses of lutein, but clinical studies to date have shown only modest and variable increases in macular carotenoid pigments in response to supplementa...Chromatography, High Pressure Liquid; Dietary Supplements; Tissue Donors2007-02
3 Digre, Kathleen B.; Warner, Judith E. A.; Bernstein, Paul S.; Alder, Stephen C.Vitamin A in the cerebrospinal fluid of patients with and without idiopathic intracranial hypertensionWe quantified vitamin A in the cerebrospinal fluid of patients with idiopathic intracranial hypertension, elevated intracranial pressure of other causes and normal intracranial pressure. Vitamin A could be detected by high-pressure liquid chromatography in most of the specimens. There was a signific...Chromatography, High Pressure Liquid; Headache2002-11
4 Blumenthal, Donald K.; Underwood, Clayton J.Adaptation of the protein kinase filter paper assay to a 96-well microtiter format.The most widely used method for assaying protein kinase activities involves incorporation of radioactive phosphate into a protein or peptide substrate with subsequent binding or precipitaion of the radiolabeled substrate onto filter paper squares. We have adapted this assay for use with readily avai...Protein Kinase Activities; Filter Papers; Radioactive Phosphate1999-02-01
5 Blumenthal, Donald K.Identification of the substrate and pseudosubstrate binding sites of phosphorylase kinase gamma-subunit.Using site-directed mutagenesis, we proposed that an autoinhibitory domain(s) is located at the C-terminal region (301-386) of the phosphorylase kinase gamma-subunit (Huang, C.-Y.F., Yuan C.-J., Livanova, N.B., and Graves, D.J. (1993) Mol. Cell. Biochem. 127/128, 7-18). Removal of the putative inhib...Mutagenesis; Autoinhibitory Domain; Peptides1995-03-31
6 Blumenthal, Donald K.Effects of deletions in the central helix of calmodulin on enzyme activation and peptide binding.Using site-directed mutagenesis we have expressed in Escherichia coli three engineered calmodulins (CaM) containing deletions in the solvent-exposed region of the central helix. These are CaM delta 84, Glu-84 removed; CaM delta 83-84, Glu-83 and Glu-84 removed; and CaM delta 81-84, Ser-81 through Gl...Drug Effects; Genetics; Metabolism1989-05-15
7 Gussin, Gary N.; Capecchi, Mario R.Protein synthesis directed by DNA phage messengers.Even through the amino acids corresponding to most of the 64 nucleotide triplets are now known, several important aspects of the genetic code are not yet fully understood. In particular we need more knowledge about the "punctuation marks" of the code-for example, the signals necessary for the initia...Carbon Isotopes; Escherichia coli; Genetic Code; Methionine1967-09-01
8 Askew, Eldon W.; Bernstein, Paul S.Serum and macular responses to antioxidant supplementation verus a carotenoid-rich dietary intervention in the elderly.The aim of this study was to observe responses of serum antioxidants, oxidative stress biomarkers, and macular carotenoid pigments to antioxidant supplements or dietary intervention in a single-masked, randomized, pilot clinical study of elderly subjects receiving antioxidant supplements or a dietar...Macula; Serum Carotenoids; Zeaxanthin2006-02-01
9 Blumenthal, Donald K.Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.Limited proteolysis has been utilized to study the structural organization of rabbit skeletal muscle myosin light chain kinase. The enzyme (Mr approximately 89,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) consists of an amino-terminal, protease-susceptible region of unidentified...Enzymology; Skeletal Muscle; Proteolysis, Peptide Fragments1995-09-15
10 Blumenthal, Donald K.Characterization of the regulatory domain of the gamma-subunit of phosphorylase kinase. The two noncontiguous calmodulin-binding subdomains are also autoinhibitory.Phosphorylase kinase is a multimeric protein kinase (alpha 4 beta 4 gamma 4 delta 4) whose enzymatic activity is conferred by its gamma-subunit. A library of 18 overlapping synthetic peptides spanning residues 277-386 of the gamma-subunit has been prepared to use in identifying important regulatory ...Metabolism; Phosphorylase Kinase1995-09-22
11 Blumenthal, Donald K.C subunits binding to the protein kinase A RI alpha dimer induce a large conformational change.We present structural data on the RI alpha isoform of the cAMP-dependent protein kinase A that reveal, for the first time, a large scale conformational change within the RI alpha homodimer upon catalytic subunit binding. This result infers that the inhibition of catalytic subunit activity is not the...Protein Kinase; cAMP2004-04-30
12 Hendee, Shonn P.; Faour, Fouad A.; Christensen, Douglas A.; Patrick, Baharah; Durney, Carl H.; Blumenthal, Donald K.Effects of weak extremely low frequency magnetic fields on calcium/calmodulin interactions.Mechanisms by which weak electromagnetic fields may affect biological systems are of current interest because of their potential health effects. Lednev has proposed an ion parametric resonance hypothesis (Lednev, 1991, Bioelectromagnetics, 12:71-75), which predicts that when the ac, frequency of a c...Electromagnetic Fields; Calcium-binding Proteins; Lednev's Theory1996-06
13 Frederick, Jeanne M.; Thulin, Craig; Bernstein, Paul S.Identification and characterization of a Pi isoform of glutathione S-transferase (GSTP1) as a zeaxanthin-binding protein in the macula of the human eyeUptake, metabolism, and stabilization of xanthophyll carotenoids in the retina are thought to be mediated by specific xanthophyll-binding proteins (XBPs). A membrane-associated XBP was purified from human macula using ion-exchange chromatography followed by gel-exclusion chromatography. Two-dimensio...Circular Dichroism; Isoelectric Focusing; Macula Lutea; Xanthophylls2004
14 Blumenthal, Donald K.Dephosphorylation of cAMP-dependent protein kinase regulatory subunit (type II) by calmodulin-dependent protein phosphatase. Determinants of substrate specificity.Calmodulin-dependent protein phosphatase purified from bovine cardiac muscle catalyzed the rapid dephosphorylation of Ser-95 of bovine cardiac cAMP-dependent protein kinase regulatory subunit (RII). The kinetic constants determined for the reaction (Km = 20 microM; Vmax = 2 mumol min-1 mg-1) are com...Metabolism; High Pressure Liquid; Enzymology1985-06-25
15 Blumenthal, Donald K.Gamma-subunit of skeletal muscle phosphorylase kinase contains two noncontiguous domains that act in concert to bind calmodulin.Phosphorylase kinase is a Ca2+-regulated, multisubunit enzyme that contains calmodulin as an integral subunit (termed the delta-subunit). Ca2+-dependent activity of the enzyme is thought to be regulated by direct interaction of the delta-subunit with the catalytic subunit (the gamma-subunit) in the ...Pharmacology; Metabolism; Enzymology1989-10-15
16 Keefe, Kristen A.Introduction to autonomic pharmacology2006-09-12
17 Blumenthal, Donald K.Ranolazine: a new antianginal agent with a novel mechanism of actionThis animation (with audio) illustrates the mechanism of action of ranolazine (RANEXA), a novel antianginal drug that inhibits the late sodium current (late I Na) in cardiac myocytes. The animation first describes how the late sodium current can contribute to symptoms of myocardial ischemia by causi...Angina; Myocardial Ischemia, Acute Coronary Syndrome; Voltage-Gated Sodium Channel, Late Sodium Current; Sodium-Calcium Exchanger; Calcium Overload; Ranolazine2006-09-19
18 Blumenthal, Donald K.Identification of molecular sites on factor VII which mediate its assembly and function in the extrinsic pathway activation complex.Factor VII-VIIa, in association with tissue factor, participates in the complex which initiates blood coagulation through the extrinsic pathway. To identify functional domains on factor VII which mediate the activation of factor X, 16 synthetic peptides corresponding to 55% of the primary structure ...Genetics; Biosynthesis; Pharmacology1991-01-15
19 Blumenthal, Donald K.; Trewhella, JillConformationally dynamic C helix of the RIalpha subunit of protein kinase A mediates isoform-specific domain reorganization upon C subunit binding.Different isoforms of the full-length protein kinase A (PKA) regulatory subunit homodimer (R2) and the catalytic (C) subunit-bound holoenzyme (R2C2) have very different global structures despite similar molecular weights and domain organization within their primary sequences. To date, it has been th...Protein Isoforms; Global Structures; cAMP2005-10-21
20 Blumenthal, Donald K.Phosphorylation of cardiac troponin by guanosine 3':5'-monophosphate-dependent protein kinase.Homogeneous cGMP-dependent protein kinase catalyzes the rapid incorporation of phosphate, specifically into the inhibitory subunit of purified cardiac troponin with a maximal incorporation of 1 mol of phosphate/mol of troponin. When troponin was incubated in the presence of both cGMP- and cAMP-depen...Protein Kinases1978-01-25
21 Blumenthal, Donald K.Rabbit skeletal muscle myosin light chain kinase. The calmodulin binding domain as a potential active site-directed inhibitory domain.A synthetic peptide modeled after the calmodulin (CaM)-binding domain of rabbit skeletal muscle myosin light chain kinase, Lys-Arg-Arg-Trp-Lys5-Lys-Asn-Phe-Ile-Ala10-Val-Ser-Ala-Ala-+ ++Asn15-Arg-Phe-Glycyl amide (M5), inhibited the CaM-independent chymotryptic fragment of the enzyme, C35 (Edelman, ...Synthetic Peptide1987-09-05
22 Veranth, John M.Computational modeling of CO/CO2 ratio inside single char particles during pulverized coal combustionA recently developed model was used to study the CO/CO2 ratio inside a burning pulverized coal particle, to better understand the effect of bulk gas composition on the equilibrium partial pressure of reduced metal species at the surface of ash inclusions. The motivation was to improve the ability to...Pulverized coal combustion; Computational modeling; Char2003
23 Blumenthal, Donald K.Characterization of the phosphotyrosyl protein phosphatase activity of calmodulin-dependent protein phosphatase.Calmodulin-dependent protein phosphatase from bovine brain and heart was assayed for phosphotyrosine and phosphoserine phosphatase activity using several substrates: 1) smooth muscle myosin light chain (LC20) phosphorylated on tyrosine or serine residues, 2) angiotensin I phosphorylated on tyrosine,...Metabolism; Phosphatase Activity1986-07-25
24 Blumenthal, Donald K.Identification of the calmodulin-binding domain of skeletal muscle myosin lightIn the course of determining the primary structure of rabbit skeletal muscle myosin light chain kinase (MLCK; ATP:protein phosphotransferase, EC 2.7.1.37) a peptide fragment was obtained that appears to represent the calmodulin-binding domain of this enzyme. Low concentrations of the peptide inhibit...Peptides; Enzymes; Protein sequence1985-05
25 Bernstein, Paul S.; Gellermann, WernerNonmydriatic fluorescence-based quantitative imaging of human macular pigment distributions.We have developed a CCD-camera-based nonmydriatic instrument that detects fluorescence from retinal lipofuscin chromophores ("autofluorescence") as a means to indirectly quantify and spatially image the distribution of macular pigment (MP). The lipofuscin fluorescence intensity is reduced at all ret...Cytology; Sensitivity and Specificity2006-10
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