Characterization of bovine rod outer segment G-protein

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Publication Type Journal Article
School or College School of Medicine
Department Ophthalmology
Creator Baehr, Wolfgang
Other Author Applebury, M. L.; Morita, E. A.; Swanson, R. J.
Title Characterization of bovine rod outer segment G-protein
Date 1982
Description A simple modified procedure is described for isolating and purifying peripherally bound membrane proteins from bovine rod outer segment disks. The methods yield milligram quantities of G-protein and cGMP phosphodiesterase which are suitable for reconstitution with membranes containing visual pigments. The properties of cGMP phosphodiesterase have been previously characterized (Baehr, W., Devlin, M. J. & Applebury, M. L. (1979) J. Biol. Chem 254, 11669-11677). Here we report the characterization and properties of the rod outer segment G-protein, a complex of three subunits a, B, and
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 257
Issue 11
First Page 6452
Last Page 6460
Subject Heterotrimeric GTP-Binding Proteins; Macromolecular Substances; Rhodopsin
Subject MESH Eye Proteins; Photoreceptors; Rod Outer Segments
Language eng
Bibliographic Citation Baehr W, Morita EA, Swanson RJ, Applebury ML. (1982). Characterization of bovine rod outer segment G-protein. J Biol Chem, 257(11), 6452-60
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Identifier ir-main,1710
ARK ark:/87278/s6zp4qqq
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706549
Reference URL https://collections.lib.utah.edu/ark:/87278/s6zp4qqq
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