Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probes

Update item information
Publication Type Poster
School or College School of Medicine
Department Pathology
Creator Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L.
Other Author Belousov, Evgeniy
Title Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probes
Date 2005-09-05
Description This poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is not possible with partial 16S rDNA sequencing and therefore requires additional testing. Conventional tests that have been used for the distinction include growth on 5% sodium chloride and growth on citrate medium, both of which have been problematic with test inaccuracies and prolonged turnaround time. Restriction analysis of an amplified fragment of the hsp65 gene can distinguish M. chelonae from M. abscessus, but this requires gel analysis and interpretation of fragment sizes and is sometimes difficult because of the similarity of restriction patterns. In this study we developed a simple and rapid method using real-time PCR with the SmartCycler TM (Cepheid, Sunnyvale, CA) to distinguish M.
Type Text
Publisher University of Utah, Associated and Regional University Pathologists (ARUP) Institute for Clinical and Experimental Pathology
Volume 43
Issue 8
First Page 4205
Last Page 4207
Subject MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus
Subject LCSH Mycobacterium; Polymerase Chain Reaction
Subject MESH Mycobacterium chelonae; Mycobacteria, Atypical; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Sensitivity and Specificity
Language eng
Bibliographic Citation Cloud, J.L., Hoggan, K., Belousov, E., Cohen, S., Brown-Elliott, B.A., Mann, L., Wilson, R., Aldous, W., Wallace, R.J. Jr., Woods, J.L. (2005). Use of the MGB Eclipse system and SmartCycler PCR for differentiation of Mycobacterium chelonae and M. abscessus. Journal of Clinical Microbiology, 43(8), 4205-7. Retrieved from http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=16081979
Rights Management http://creativecommons.org/licenses/by-nc-nd/2.5/
Format Medium application/pdf
Format Extent 1,950,498 bytes (1.85 MB)
Identifier ir-main,241
ARK ark:/87278/s6sb4q9b
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-11-30
ID 706299
Reference URL https://collections.lib.utah.edu/ark:/87278/s6sb4q9b
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