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CreatorTitleDescriptionSubjectDate
1 Dwight, Zachary LawrenceuAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictionsAbstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce...2012-01-01
2 Dwight, Zachary LawrenceHeterozygote PCR product melting curve predictionMelting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four...2014-01-01
3 Planelles, VicenteKinase control of latent HIV-1 infection: PIM-1 kinase as a major contributor to HIV-1 reactivationDespite the clinical relevance of latent HIV-1 infection as a block to HIV-1 eradication, the molecular biology of HIV-1 latency remains incompletely understood. We recently demonstrated the presence of a gatekeeper kinase function that controls latent HIV-1 infection. Using kinase array analysis w...2014-01-01
4 Slev, Patricia R.; Rawlins, Mindy, L.; Roberts, William L.Performance characteristics of seven automated CA 15-3 methodsPoster describing studies to evaluate seven automated methods for the following parameters: limit of detection, linearity, imprecision, reference intervals and method comparison with the ADVIA Centaur as the comparison method to detect CA 15-3.CA 15-3 Method2005-07-13
5 Wittwer, Carl T.Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instrumentsBACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us...2014-01-01
6 Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V.Cross platform comparison of melting curve analysisMelting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt...PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping2005-11-04
7 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R; Hillyard, David RComparison of the DIGENE HBV HYBRID CAPTURE II AND ROCHE COBAS Amplicor HBV Monitor Assays.Evaluate and compare the performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics Corp.) and the HBV Hybrid capture II (Digene Corporation) assay for the quantification of HBV BNA Levels.Quantitative Tests; Linearity Studies2001-08-19
8 Normann, Richard A.; Chandler, John P.Effects of calcium ions on L-type horizontal cells in the isolated turtle retinaA technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p...Retina; Calcium; Horizontal Cell; Turtle1990
9 Jaskowski, Troy D.Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination controlToxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction...PCR probe capture assay; Uracil N-glycosylase; UNG2000
10 Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M.Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysisDifferentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar...MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR2006-01-09
11 Rockwood, Alan L.Mass spectrometry for steroids, immunosuppressants, and other clinical applicationsPresentation slides on matching mass spectrometry technology to clinical applications.Immunosuppressants2005-07-26
12 Owen, William E.; Roberts, William L.Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluidMeasurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA an...IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval2003
13 Jaskowski, Troy D.Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassayWe used a multiplexed fluorescent microsphere immunoassay to develop a sandwich capture assay to assess simultaneously the production of thymus helper (TH) 1- and TH2-type cytokines in tissue culture supernatant obtained from stimulated peripheral blood mononuclear cells. The assay then was use...Multiplexed fluorescent microsphere immunoassay2002
14 Hymas, Weston C.Ultrasonic enhancement of antibiotic action on several species of bacteriaThe effect of the antibiotics gentamicin, streptomycin, kanamycin, tetracycline, and ampicillin on planktonic cultures of Enterobacter aerogenes, Serratia marcescens, Salmonella derby, Streptococcus mitis, and Staphylococcus epidermidis with and without an application of 70 kHz ultrasound was studi...Enterobacter aerogenes; Serratia marcescens; Salmonella derby; Streptococcus mitis; Staphylococcus epidermidis; Planktonic cultures; Gentamicin; Streptomycin; Kanamycin; Tetracycline; Ampicillin; Ultrasonic enhancement; Bioacoustic effect1998
15 Erickson, J. Alan; Ashwood, Edward R.Evaluation of an enzyme-linked binding protein assay for hyaluronic acid and concentrations in hepatitis C infected patientsSerological hyaluronic acid (HA) has been proposed as a noninvasive alternative to liver biopsy for assessing the extent of liver fibrosis. Liver biopsy correctly identifies hepatic disease in about 65 to 75% of cases, being strongly dependent on the length of the biopsy obtained. Furthermore, hepat...Hyaluronic Acid Concentration; Corgenix HA assay2005-07-21
16 Erickson, J. Alan; Aldeen, William E.; Ashwood, Edward R.Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: assessment versus an established assay and implication in classifying pancreatic functionDisagreement continues regarding the two commercially available fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function. Our objectives were to validate the newer PE-1 ELISA and evaluate the test against the previously established assay, to investiga...PE-1; pancreatic elastase-1; FDA; AMR; analytical measurement range; BioServ Diagnostics PE-1ELISA; ScheBo Biotech PE-1ELISA2005-09-25
17 Erickson, J. Alan; Smith, Jeffrey, J.; Bornhorst, Joshua A.; Ashwood, Edward R.Humananti-mouse antibody protected ELISA for the quantification of squamous cell carcinoma antigenSquamous cell carcinoma antigen (SCC) was first isolated from squamous cell carcinoma tissue of the uterine cervix and initially reported as TA-4. The antigen has been characterized as a glycoprotein with a molecular weight between 45,000 and 55,000 daltons. Studies suggest that SCC may function as ...SCC; Squamous Cell Carcinoma Antigen; BSA; PBS; Phosphate Buffered Saline; HAMA; Human Anti-mouse Antibodies; IIR; ImmunoglobulinInhibiting Reagent2005-12-09
18 Owen, William E.; Roberts, William L.Comparison of five automated serum and whole blood folate assaysSerum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, wer...Folate assays; Serum folate; Whole blood folate; Automated assays2003
19 Litwin, Christine M.Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis diseaseAnti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s...2012-01-01
20 Aldous, Jay A.; Engar, Richard C.Do dentists prescribe narcotics excessively?Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ...Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative1996-07-01
21 Mongia, Shella K.; Rawlins, Mindy, L.; Owen, William E.; Roberts, William L.Performance characteristics of seven automated CA125 assaysAuthor manuscript. Cancer antigen 125 (CA125) is a high molecular mass glycoprotien, which is used as tumor marker to monitor disease progression, response to therapy and in early detection of recurrence after treatment for ovarian cancer. The Access 2, ADVIA Centaur, ARCHITECT i2000, AxSYM, Elecsys...CA125 assays2005-08-29
22 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.; Comanor, Lorraine; Di Canzio, JamesEvaluation of the Versant ? HBV DNA 3.0 Assay (bDNA)+ and comparison with the Cobastm HBV Taqman Analyte Specific Reagent Assay.To evaluate the performance characteristics of the Versant ? HBV DNA 3.0 assay (bDNA)+ (Versant, Bayer Healthcare LLC, Tarrytown, NY). 2.) To compare the Versant assay to the COBAS HBV TaqMan Analyte Specific Reagent assay (TaqMan) (Roche Molecular Systems, Pleasanton, CA) using random and defined p...International Standard; HBV DNA Tests2005-05-07
23 Roberts, William L.Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principlesStandardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances.2012
24 Owen, William E.; Roberts, William L.Performance characteristics of an HPLC method for Urinary AlbuminMicroalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti...MA; Microalbuminuria; HPLC2005-07-06
25 Owen, William E.; Roberts, William L.Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods.Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur...Glycohemoglobin; GHB; Glycated Hb; Glycemic control2008
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