Novel one step real time RT-PCR Assay for the detection of Enterovirus

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Publication Type Poster
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Hymas, Weston C.; Hillyard, David R.
Title Novel one step real time RT-PCR Assay for the detection of Enterovirus
Date 2005-07-11
Description nteroviruses (EV) are the leading cause of aseptic meningitis in pediatric and adult populations and can be associated with severe disease such as myocarditis, encephalitis, and paralytic poliomyelitis. RT-PCR has rapidly become the diagnostic methodology of choice due to its sensitivity and rapid turn-around-time allowing significant improvement in patient care and management. Most molecular assays target the highly conserved regions within the 5' nontranslated region (NTR) described by Rotbart el al. We describe the development of a novel real time assay that amplifies and detects a conserved region upstream of the Rotbart amplicon utilizing primers and an Eclipse probe. As a further enhancement, an RNA internal control (IC) is integrated into the reaction. The analytical sensitivity was determined and a comparison to the Chemicon Oligodetect Panenterovirus kit was made during the 2005 enterovirus season.
Type Text
Publisher University of Utah
Subject Viruses; Isolation; Purification
Subject MESH Chemistry, Analytical; Clinical Laboratory Techniques; Immunoassay; Virus Diseases
Language eng
Bibliographic Citation Hymas WC, Hillyard DR. A Novel One Step Real Time RT-PCR Assay for the Detection of Enterovirus.
Format Medium application/pdf
Identifier ir-main,508
ARK ark:/87278/s6fn1qr1
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706172
Reference URL
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