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Creator | Title | Description | Subject | Date |
| 1 |
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| Autosomal dominant cerebellar ataxia with retinal degeneration: clinical, neuropathologic, and genetic analysis of a large kindred. | The autosomal dominant cerebellar ataxias (ADCA) comprise a heterogeneous group of neurologic disorders characterized by degeneration of the cerebellum, spinal cord, and brainstem. Genetic analysis has revealed two loci, SCA1 on chromosome 6p, and SCA2 on chromosome 12q, responsible for some ADCA. W... | Retinal Degeneration; Cerebellar Ataxia; Genetic Analysis | 1994-08 |
| 2 |
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| Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometry | Background: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show... | | 2014-01-01 |
| 3 |
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| Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principles | Standardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances. | | 2012 |
| 4 |
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| Performance characteristics of an HPLC method for Urinary Albumin | Microalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti... | MA; Microalbuminuria; HPLC | 2005-07-06 |
| 5 |
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| Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods. | Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur... | Glycohemoglobin; GHB; Glycated Hb; Glycemic control | 2008 |
| 6 |
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| Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis [abstract] | This abstract describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the meth... | Genotyping; Amplicon Melting | 2005-06-22 |
| 7 |
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| Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids | Congenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O... | Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay | 2006 |
| 8 |
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| Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis | This poster describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the method... | Genotyping; Amplicon Melting | 2005-06 |
| 9 |
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| Novel one step real time RT-PCR assay for the detection of Enterovirus, A | This poster describes the development of a novel real time assay that utilizes primers and an Eclipse probe in an overlapping format upstream of the Rotbart amplicon. | RT-PCR Assay | 2005-11-08 |
| 10 |
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| Real time RT-PCR assay for norovirus detection with eclipse probes and a non-competitive internal control. | Noroviruses are the major causative agents of nonbacterial gastroenteritis worldwide and are estimated to cause approximately 23 million cases in the United States annually. Real time RT-PCR is rapidly becoming the principle diagnostic means for norovirus detection due to its enhanced sensitivity an... | Gastroenteritis; Eclipse Hybridization Probes; Norovirus | 2005-05-03 |
| 11 |
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| Novel one step real time RT-PCR Assay for the detection of Enterovirus | nteroviruses (EV) are the leading cause of aseptic meningitis in pediatric and adult populations and can be associated with severe disease such as myocarditis, encephalitis, and paralytic poliomyelitis. RT-PCR has rapidly become the diagnostic methodology of choice due to its sensitivity and rapid t... | Viruses; Isolation; Purification | 2005-07-11 |
| 12 |
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| Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) Assay and Comparison to the COBAS Amplicor HBV Monitor Assay. | erformance characteristics of the COBAS HBV TaqMan ASR (TaqMan) assay were evaluated and compared to the COBAS Amplicor HBV Monitor test (Amplicor) assay for the quantification of HBV DNA in clinical samples. | Quantitative Tests; Linearity Studies | 2004-05-03 |
| 13 |
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| Single tube real-time RT-PCR Assay for the detection of enterovirus | We developed a rapid and sensitive method for the routine detection of enterovirus in CSF and plasma using a one tube, one step, real-time PCR assay. A primer set and an Eclipse probe were designed to target the highly conserved 5' untranslated region of the enterovirus genome. This assay was compar... | MGB Eclipse; Viral RNA; Picornaviridae | 2004-09-11 |
| 14 |
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| Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay. | To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international... | Quantitative Tests; Linearity Studies | 2004-05-10 |
| 15 |
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| Performance Characteristics of the COBAS HCV TaqMan ASR Using Armored RNA Calibrators | As improved therapies have become available for Hepatititis C Virus (HCV) infection, the use of assays to quantitate HCV RNA has increased dramatically (3, 14, 16). The initial use of these assays was to predict the likelihood of therapy based on baseline HCV RNA levels. These reports indicated that... | Roche Amplicor Monitor; NGI Superquant; patients | 2003-04-22 |
| 16 |
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| Performance characteristics of the COBAS HCV TaqMan ASR and Comparison to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0. | Evaluate the COBAS HCV TaqMan ASR assay using both the Qiagen BioRobot 9604 and a 1mL extraction protocol for HCV RNA isolation. And Compare the COBAS TaqMan HCV ASR assay to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0 assays. | Quantitative Tests; Linearity Studies | 2002-11-21 |
| 17 |
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| Quantitative real time PCR assay for the detection of human herpes 6. | Human herpes virus type 6 is a beta virus that occurs as two variants (A & B) and infects nearly 100% of the population before two years of age. Primary infections in children can produce fever and a unique lacy rash that gives the disease its name: roseola. The rare primary infection in adults can ... | Probe Technology; Roseola Infantum | 2005-02-05 |
| 18 |
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| Eclipse probe assay for the detection of mycoplasma pneumoniae. | Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ... | Eclipse Hybridization Probe; Protocol; Techniques | 2005-09-11 |
| 19 |
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| Masking selected sequence variation by incorporating mismatches into melting analysis probes | Hybridization probe melting analysis can be complicated by the presence of sequence variations (benign polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Selected sequence variati... | probe hybridization; high-resolution melting analysis; masking; RET | 2005-06-28 |
| 20 |
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| Quantitative real time PCR assay for detecting EBV virus in multiple sample types. | We are developing a real time quantitative PCR assay to detect EBV in serum, plasma, whole blood, tissue and spinal fluid. Real time PCR, with its intrinsic quantitative capacity, is an excellent method for measuring EBV viral load. Epstein Barr virus is a member of the Herpesvirus family, with a tr... | Eclipse Probes; EBV Primer | 2005-08-11 |
| 21 |
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| Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments | BACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us... | | 2014-01-01 |
| 22 |
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| uAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictions | Abstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce... | | 2012-01-01 |
| 23 |
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| Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluid | Measurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA an... | IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval | 2003 |
| 24 |
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| Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassay | We used a multiplexed fluorescent microsphere immunoassay to develop a sandwich capture assay to assess simultaneously the production of thymus helper (TH) 1- and TH2-type cytokines in tissue culture supernatant obtained from stimulated peripheral blood mononuclear cells. The assay then was use... | Multiplexed fluorescent microsphere immunoassay | 2002 |
| 25 |
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| Effects of calcium ions on L-type horizontal cells in the isolated turtle retina | A technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p... | Retina; Calcium; Horizontal Cell; Turtle | 1990 |
