Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluid

Update item information
Publication Type Journal Article
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Owen, William E.; Roberts, William L.
Title Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluid
Date 2003
Description Measurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA and IgM tests on the IMMAGE Immunochemistry System were evaluated for linearity, imprecision, method comparison, and reference interval verification. Both IgA methods were linear from 1.4 to at least 50 mg/L. Both IgM methods were linear from 0.14 to more than 6 mg/L. The total imprecision of the BN II IgA and IgM methods and the IMMAGE IgA method was less than 10%. The imprecision of the IMMAGE IgM method was 10.2% at 0.49 mg/L and less than 5% at higher IgM concentrations. Method comparison studies indicated that IgA and IgM methods on both instruments showed good comparability. Reference interval studies demonstrated that both methods had similar reference intervals that agreed with published values of less than 6 mg/L for IgA and less than 1.3 mg/L for IgM. Methods for quantifying IgA and IgM in CSF on the BN II and IMMAGEnephelometers perform well and give comparable results.
Type Text
Publisher American Society for Clinical Pathology
Volume 119
Issue 5
First Page 689
Last Page 693
Subject IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval
Subject LCSH Immunoglobulin A -- Measurement; Immunoglobulin M -- Measurement; Multiple sclerosis -- Diagnosis; Immunoassay; Cerebrospinal fluid
Language eng
Bibliographic Citation Owen, W. E., & Roberts, W. L. (2003). Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluid. American Journal of Clinical Pathology, 119(5), 689-93.
Rights Management ©2003-2008 American Society for Clinical Pathology and ©2003-2008 ASCP Press
Format Medium application/pdf
Format Extent 79,228 bytes
Identifier ir-main,11557
ARK ark:/87278/s6m33d8t
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706016
Reference URL https://collections.lib.utah.edu/ark:/87278/s6m33d8t
Back to Search Results