Hypericin: a potential antiglioma therapy

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Publication Type Journal Article
School or College School of Medicine
Department Neurosurgery
Creator Couldwell, William T.
Other Author Gopalakrishna, Rayudu; Hinton, David R.; He, Shikun; Weiss, Martin H.; Law, Ronald E.; Apuzzo, Michael L. J.
Title Hypericin: a potential antiglioma therapy
Date 1994
Description HYPERICIN, A POLYCYCLIC aromatic dione isolated from plants, is presently being clinically evaluated as an antiviral agent in the treatment of human immunodeficiency virus (HIV) infection. In addition, it is known to be a potent protein kinase C inhibitor. To evaluate its potential as an inhibitor of glioma growth, an established (U87) and low-passage glioma line (93-492) were treated with hypericin in tissue culture for a period of 48 hours after passage. Hypericin inhibited the glioma growth in a dose-related manner, with a marked inhibition of growth in the low-micromolar concentration range (e.g., in line U87 and low-passage line 93-492 , a concentration of hypericin of 10 pmol/L produced 62 and 76% decreases in [3H]thymidine uptake, respectively). Because the reported inhibitory effects of protein kinase Care enhanced by visible light, [3H]thymidine uptake was measured in both the presence and the absence of visible light. In glioma line A172, the presence of light slightly increased the inhibitory effect of hypericin. Moreover, an apoptosis (i.e., programmed cell death) assay was performed to determine whether the treatment of glioma cells with hypericin was cytostatic or cytocidal. Cells were harvested, and purified deoxyribonucleic acid (DNA) was analyzed by agarose gel electrophoresis. DNA from cells treated with hypericin for 48 hours exhibited a classical "ladder" pattern of oligonucleosome-sized fragments characteristic of apoptosis. These data suggest that the proven safe drug hypericin may have potential as an antiglioma agent; we suggest clinical trials.
Type Text
Publisher Wolters Kluwer (LWW)
Volume 35
Issue 4
First Page 705
Last Page 710
Subject Hypericin; Protein kinase C; Glioma; Brain neoplasm
Subject LCSH Gliomas -- Treatment; Protein kinases; Apoptosis; Brain -- Cancer
Language eng
Bibliographic Citation Couldwell, W. T., Gopalakrishna, R., Hinton, D. R., He, S., Weiss, M. H., Law, R. E., & Apuzzo, M. L. J. (1994). Hypericin: a potential antiglioma therapy. Neurosurgery, 35(4), 705-10.
Rights Management (c) Wolters Kluwer (LWW) http://lww.com
Format Medium application/pdf
Format Extent 854,217 bytes
Identifier ir-main,12538
ARK ark:/87278/s6p565tj
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 704442
Reference URL https://collections.lib.utah.edu/ark:/87278/s6p565tj
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