Novel model of intracranial meningioma in mice using luciferase-expressing meningioma cells

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Publication Type Journal Article
School or College School of Medicine
Department Neurosurgery
Creator Couldwell, William T.; Gillespie, David L.; Jensen, Randy L.
Other Author Ragel, Brian T.; Elam, Isaac L.; Flynn, Jeannette R.; Kelly, David A.; Mabey, David; Feng, Harvey
Title Novel model of intracranial meningioma in mice using luciferase-expressing meningioma cells
Date 2008-01-01
Description Object. Meningioma research has been hindered by the inability to sequentially measure intracranial tumor growth in a cost-effective, efficient manner. Recently, the luciferase gene has been transfected into cancer lines to obtain cells that express the luciferase enzyme, which oxidizes luciferin in a reaction that releases photon energy that can be measured noninvasively by bioluminescence imaging (BLI) systems. The authors describe a mouse model of intracranial meningioma that uses this novel BLI system. Methods. The immortal meningioma cell lines CH-157-MN and IOMM-Lee were transfected with luciferase and neomycin phosphotransferase (LucNeo) and selected with G418. These cells were stereotactically implanted at skull base and cerebral convexity locations in nude mice. Animals were imaged for bioluminescence biweekly, and 5 mice underwent magnetic resonance (MR) imaging. Tumors were harvested for immunohistochemical and ultrastructural analysis. Results. The CH-157-MN-LucNeo and IOMM-Lee-LucNeo cell lines were successfully implanted intracranially in mice. The tumor induction rate for CH-157-MN-LucNeo skull base tumors was 90% (36 of 40 procedures). Statistical analysis of CH-157-MN-LucNeo skull base tumor volume measured on MR imaging correlated with the results of BLI showed an R value of 0.900. The tumors exhibited characteristics of aggressive meningiomas by insinuating along arachnoid planes and invading brain. Conclusions. Noninvasive BLI was successfully used to image intracranial meningiomas in mice. The tumors grew in a fashion similar to that of aggressive meningiomas in humans, and exhibited the microscopic, immunohistochemical, and ultrastructural features characteristic of meningiomas. This animal model overcomes the main obstacle in studying intracranial meningiomas by enabling sequential noninvasive tumor measurement in a cost-effective manner.
Type Text
Publisher American Association of Neurological Surgeons (AANS)
Volume 108
Issue 2
First Page 304
Last Page 310
Language eng
Bibliographic Citation Ragel, B. T., Elam, I. L., Gillespie, D. L., Flynn, J. R., Kelly, D. A., Mabey, D., Feng, H., Couldwell, W. T., & Jensen, R. L. (2008). Novel model of intracranial meningioma in mice using luciferase-expressing meningioma cells. Laboratory investigation. Journal of Neurosurgery, 108(2), 304-10.
Rights Management (c) American Association of Neurological Surgeons
Format Medium application/pdf
Format Extent 550,939 bytes
Identifier ir-main,12656
ARK ark:/87278/s6nz8rp5
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 702363
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