Selective degradation of abnormal proteins in mammalian tissue culture cells.

Update Item Information
Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Capecchi, Nancy E.: Hughes, Stephen H.; Wahl, Geoffrey M.
Title Selective degradation of abnormal proteins in mammalian tissue culture cells.
Date 1974-12-01
Description The degradation rates of several missense mutants of hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) in mouse L cells are compared to those of the wild-type enzyme. Although the rates of total protein breakdown in the mutant cell lines are identical to that of the parental L cell line, defective molecules of hypoxanthine-guanine phosphoribosyltransferase present in the mutant cell lines are degraded much faster than the wild-type enzyme. The level of defective phosphoribosyltransferase molecules present in the mutant cell lines is inversely proportional to the breakdown rate. This observation indicates that the major factor determining the concentrations of the defective phosphoribosyltransferases is their specific degradation rate. These results strongly support the hypothesis that abnormal proteins are selectively degraded in mammalian cells.
Type Text
Publisher National Academy of Sciences of the United States of America.
Volume 71
Issue 12
First Page 4732
Last Page 4736
Subject Gene Expression Regulation; Mice, Transgenic; Microscopy, Fluorescence
Subject MESH RNA, Messenger; Ribonucleoproteins; Spermatids; Spermatogenesis
Language eng
Bibliographic Citation Proc Natl Acad Sci U S A. 1974 Dec;71(12):4732-6: Capecchi MR, Capecchi NE, Hughes SH, Wahl GM. Selective degradation of abnormal proteins in mammalian tissue culture cells. Retrieved on September 22.2006 from http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=4531013.
Rights Management Copyright © 1974 National Academy of Sciences of the United States of America. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,464
ARK ark:/87278/s6x92vzn
Setname ir_uspace
ID 707417
Reference URL https://collections.lib.utah.edu/ark:/87278/s6x92vzn