Publication Type |
pre-print |
School or College |
College of Science |
Department |
Biology |
Creator |
Hughes, Kelly T. |
Other Author |
Galeva, Anna; Moroz, Natalia; Yoon, Young-Ho; Samatey, Fadel A.; Kostyukova, Alla S. |
Title |
Bacterial flagellin-specific chaperone FliS interacts with anti-sigma factor FlgM |
Date |
2014-01-01 |
Description |
Flagella are extracellular organelles that propel bacteria. Each flagellum consists of a basal body, a hook, and a filament. The major protein of the filament is flagellin. To prevent premature polymerization of newly synthesized flagellin molecules, FliS, the flagellin-specific chaperone, binds flagellin and facilitates its export. Induction of flagellin gene expression coincides with secretion of FlgM. The role of FlgM is to inhibit FliA (Ơ28), a flagellar-specific RNA polymerase responsible for flagellin transcription. To prevent premature polymerization of newly synthesized flagellin molecules, FliS, the flagellin-specific chaperone, binds flagellin and facilitates its export. In this study, the interaction between FlgM and FliS from Salmonella typhimurium was characterized using gel-shift, intrinsic tryptophan fluorescence, circular dichroism, limited proteolysis and cross-linking. We have demonstrated that: i) FliS and FlgM interact specifically, forming a 1:1 complex; ii) The FliS binding site on FlgM is proximal to or even overlaps with the binding site for FliA; and iii) FliA competes with FliS for FlgM binding. We suggest that FliS binding by FlgM helps to stabilize and preserve FlgM in the cell until FliA is expressed. |
Type |
Text |
Publisher |
American Society for Microbiology |
Volume |
196 |
Issue |
6 |
First Page |
1215 |
Last Page |
1221 |
Language |
eng |
Bibliographic Citation |
Galeva, A., Moroz, N., Yoon, Y.-H., Hughes, K. T., Samatey, F. A., & Kostyukova, A. S. (2014). Bacterial flagellin-specific chaperone FliS interacts with anti-sigma factor FlgM. Journal of Bacteriology, 196(6), 1215-221. |
Rights Management |
(c)American Society for Microbiology ; doi: 10.1128/JB.01278-13. |
Format Medium |
application/pdf |
Format Extent |
2,239,983 bytes |
Identifier |
uspace,18515 |
ARK |
ark:/87278/s6rc0dqh |
Setname |
ir_uspace |
ID |
712837 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6rc0dqh |