Comparison of five automated serum and whole blood folate assays

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Publication Type Journal Article
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Owen, William E.; Roberts, William L.
Title Comparison of five automated serum and whole blood folate assays
Date 2003
Description Serum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, were performed with the Access, Advia Centaur, ARCHITECT i2000, Elecsys 2010, and IMMULITE 2000 methods. The QuantaPhase II radioassay served as the comparison method. (Proprietary information is given in the text.) The Access and IMMULITE 2000 methods had higher systematic errors in linearity studies than the other 3 methods. The imprecision of all methods was acceptable (coefficient of variation, <10%) even at low folate concentrations with the exception of the Elecsys 2010 (coefficient of variation, 16%). Method comparison studies using serum samples revealed calibration differences between the Access and Elecsys 2010 methods and the comparison method. Method comparison studies using whole blood samples showed poorer agreement between each of the automated methods and the comparison method than was seen with serum samples. The ARCHITECT i2000 folate assay demonstrated the best analytic performance. The poor agreement seen with whole blood hemolysates likely is due to calibration differences and differences in hemolysate preparation conditions.
Type Text
Publisher American Society for Clinical Pathology
Volume 120
Issue 1
First Page 121
Last Page 126
Subject Folate assays; Serum folate; Whole blood folate; Automated assays
Subject LCSH Folic acid deficiency -- Measurement
Language eng
Bibliographic Citation Owen, W. E., & Roberts, W. L. (2003). Comparison of five automated serum and whole blood folate assays. American Journal of Clinical Pathology, 120(1), 121-6.
Rights Management ©2003-2008 American Society for Clinical Pathology and ©2003-2008 ASCP Press
Format Medium application/pdf
Format Extent 126,359 bytes
Identifier ir-main,11556
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Date Created 2012-06-13
Date Modified 2021-05-06
ID 702759
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