Cross platform comparison of melting curve analysis

Melting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melting analysis for genotyping and screening. With the gaining acceptance of melting analysis as a diagnostic tool, there is a need to characterize the ability of commercially available real-time PCR instruments to perform melting analyses. In the current study, a systematic evaluation of melting analysis capability was undertaken employing eight real-time machines from six vendors in resolving SNP genotypes by melting with SYBR Green I and LCGreen Plus.