| Publication Type |
Journal Article |
| School or College |
College of Science; School of Medicine |
| Department |
Oncological Sciences; Biology; Human Genetics |
| Program |
Institute of Human Genetics; Howard Hughes Medical Institute (HHMI) |
| Creator |
Capecchi, Mario R. |
| Other Author |
Hughes, Stephen H.; Wahl, Geoffrey M. |
| Title |
Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase. |
| Date |
1975-01-31 |
| Description |
Hypoxanthine-guanine phosphoribosyltransferase (HGPR transferase) (EC 2.4.2.8) has been purified approximately 4500-fold to apparent homogeneity from mouse liver. The procedure involves the use of affinity chromatography and was designed to be readily adaptable to small scale isolations. The enzyme appears to be composed of 3 subunits of identical molecular weight (27,000 per subunit). The subunit molecular weight has also been determined by the analysis of radioactively labeled HGPR transferase immunoprecipitated from wild type and mutant (HGPR transferase) mouse tissue culture cell lines. |
| Type |
Text |
| Publisher |
American Society for Biochemistry and Molecular Biology (ASBMB) |
| Volume |
250 |
| Issue |
1 |
| First Page |
120 |
| Last Page |
126 |
| Subject |
Buffers; Centrifugation, Density Gradient; Chromatography, Affinity; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel |
| Subject MESH |
Guanine Nucleotides; Liver; Pentosyltransferases; Sulfur Radioisotopes; Ultrafiltration |
| Language |
eng |
| Bibliographic Citation |
J Biol Chem. 1975 Jan 10;250(1):120-6: Hughes SH, Wahl GM, Capecchi MR. Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase. Retrieved on September 22.2006 from http://www.jbc.org/cgi/reprint/250/1/120. |
| Rights Management |
Copyright © 1975 American Society for Biochemistry and Molecular Biology. All Rights Reserved.. |
| Format Medium |
application/pdf |
| Identifier |
ir-main,462 |
| ARK |
ark:/87278/s6ng585p |
| Setname |
ir_uspace |
| ID |
706124 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6ng585p |
