Efficient shine- Dalgarno sequence but not translation is necessary for LacZ mRNA stability in Escherichia coli

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Publication Type Journal Article
School or College School of Medicine; College of Science; College of Engineering
Department Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
Creator Gesteland, Raymond F.; Weiss, Robert B.
Other Author Wagner, Lori A.; Dayhuff, Timothy J.
Title Efficient shine- Dalgarno sequence but not translation is necessary for LacZ mRNA stability in Escherichia coli
Date 1994
Description The 5' ends of many bacterial transcripts are important in determining mRNA stability. A series of Shine-Dalgarno (SD) sequence changes showed that the complementarity of the SD sequence to the anti-SD sequence of 16S rRNA correlates with lacZ mRNA stability in Escherichia coli. Several initiation codon changes showed that an efficient initiation codon is not necessary to maintain lacZ mRNA stability. A stop codon in the 10th codon of lacZ increased mRNA stability. Therefore, ribosomal binding via the SD sequence but not translation of the coding region is necessary to maintain lacZ mRNA stability.
Type Text
Publisher American Society for Microbiology
First Page 1683
Last Page 1688
Subject Protein Biosynthesis; Ribosomes; Escherichia coli; Shine-Dalgarno Sequence
Subject MESH Molecular Sequence Data; Mutation; Protein Biosynthesis
Language eng
Bibliographic Citation Wagner, L.A., Gesteland, R.F., Dayhuff, T.J. and Weiss, R.B. 1994. An efficient Shine- Dalgarno sequence but not translation is necessary for LacZ mRNA stability in Escherichia coli. J. Bact. 176:1683-1688
Rights Management ©American Society for Microbiology
Format Medium application/pdf
Identifier ir-main,12774
ARK ark:/87278/s6n87tz9
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 703280
Reference URL https://collections.lib.utah.edu/ark:/87278/s6n87tz9