rRNA-mRNA base pairing stimulates a programmed -1 ribosomal frameshift

Update Item Information
Publication Type Journal Article
School or College School of Medicine; College of Science; College of Engineering
Department Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
Creator Gesteland, Raymond F.; Atkins, John F.; Wills, Norma M.
Other Author Larsen, Bente
Title rRNA-mRNA base pairing stimulates a programmed -1 ribosomal frameshift
Date 1994
Description Base pairing between the 3' end of 16S rRNA and mRNA is shown to be important for the programmed -1 frameshifting utilized in decoding the Escherichia coli dnaX gene. This pairing is the same as the Shine-Dalgarno pairing used by prokaryotic ribosomes in selection of translation initiators, but for frameshifting the interaction occurs within elongating ribosomes. For dnaX -1 frameshifting, the 3' base of the Shine-Dalgarno sequence is 10 nucleotides 5' of the shift site. Previously, Shine-Dalgarno rRNA-mRNA pairing was shown to stimulate the +1 frameshifting necessary for decoding the release factor 2 gene. However, in the release factor 2 gene, the Shine-Dalgarno sequence is located 3 nucleotides 5' of the shift site. When the Shine-Dalgarno sequence is moved to the same position relative to the dnaX shift site, it is inhibitory rather than stimulatory. Shine-Dalgarno interactions by elongating ribosomes are likely to be used in stimulating -1 frameshifting in the decoding of a variety of genes.
Type Text
Publisher American Society for Microbiology
First Page 6842
Last Page 6851
Subject Frameshifting; Escherichia coli; Binding Sites; Base Composition
Subject MESH Molecular Sequence Data; Sequence Deletion; RNA, Double-Stranded; DNA Mutational Analysis
Language eng
Bibliographic Citation Larsen, B., Wills, N.M., Gesteland, R.F., and Atkins, J.F. 1994. rRNA-mRNA base pairing stimulates a programmed -1 ribosomal frameshift. J. Bacteriol. 176:6842-6851
Rights Management ©American Society for Microbiology
Format Medium application/pdf
Identifier ir-main,21773
ARK ark:/87278/s6mg874n
Setname ir_uspace
ID 706721
Reference URL https://collections.lib.utah.edu/ark:/87278/s6mg874n