Activation of Src family kinases in spinal microglia contributes to formalin-induced persistent pain state through p38 pathway

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Publication Type pre-print
School or College School of Medicine
Department Anesthesiology
Creator Light, Alan R.
Other Author Tan, Yong-Hui; Li, Kai; Chen, Xin-Yi.; Cao, Ye; Fu, Kai-Yuan
Title Activation of Src family kinases in spinal microglia contributes to formalin-induced persistent pain state through p38 pathway
Date 2012-01-01
Description Protein tyrosine phosphorylation has been implicated in normal and pathological functions, such as cell proliferation, migration and differentiation. Recently, some studies have shown that Src family kinases (SFKs) were involved in neurological disorders and neuropathic pain states in which microglial activation plays a role. In the formalin test, we have reported that microglia undergo at least two distinct stages of activation on the basis of signaling events regarding p38 mitogen-activated protein kinases (MAPK). Here, we investigated the involvement of SFKs signaling in formalin pain animal model, and the association with p38 MAPK. Our results showed that SFKs were activated in the spinal microglia beginning 1 day after peripheral formalin injection, lasting for 7 days. Pretreatment with SFK specific inhibitor PP2 could not inhibit formalin induced spontaneous pain behaviors. However, PP2 inhibited formalin injury induced persistent mechanical hyperalgesia, and reversed microglial phospho-p38 expression as well using immunohistostaining and Western blot at day 3 and 7 after injection. Our results suggested that the activation of the Src/p38MAPK signaling cascade in spinal microglia contributed to late stage persistent mechanical hyperalgesia evoked by formalin injection into the paw.
Type Text
Publisher Elsevier
Volume 13
Issue 10
First Page 1008
Last Page 1015
Dissertation Institution University of Utah
Language eng
Bibliographic Citation Tan, Y.-H., Li, K., Chen, X.-Y., Cao, Y., Light, A.;R., & Fu, K.-Y. (2012). Activation of Src family kinases in spinal microglia contributes to formalin-induced persistent pain state through p38 pathway. Journal of Pain, 13(10), 1008-15.
Rights Management (c) Elsevier; Reprinted from Tan, Y.-H., Li, K., Chen, X.-Y., Cao, Y., Light, A.;R., & Fu, K.-Y. (2012). Activation of src family kinases in spinal microglia contributes to formalin-induced persistent pain state through p38 pathway. Journal of Pain, 13(10), 1008-15. http://dx.doi.org/DOI: 10.1016/j.jpain.2012.07.010.
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Identifier uspace,17975
ARK ark:/87278/s6k36cfg
Setname ir_uspace
Date Created 2013-02-08
Date Modified 2013-02-08
ID 708316
Reference URL https://collections.lib.utah.edu/ark:/87278/s6k36cfg