Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Deng, Chuxia
Title Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus.
Date 1992-08-12
Description Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22 different sequence replacement and sequence insertion vectors. The targeting frequency was examined at two sites within the Hprt locus as a function of the extent of homology between the targeting vector and the target locus. The targeting frequency was also compared by using vectors prepared from isogenic and nonisogenic DNA sources. With one exception, all of the vectors showed the same exponential dependence of targeting efficiency on the extent of homology between the targeting vector and the target locus. This was true regardless of whether they were sequence replacement or sequence insertion vectors, whether they were directed toward either of the two different sites within the Hprt locus, or whether they were prepared from isogenic or nonisogenic DNA sources. Vectors prepared from isogenic DNA targeted four to five times more efficiently than did the corresponding vectors prepared from nonisogenic DNA. The single case of unexpectedly low targeting efficiency involved one of the vectors prepared from nonisogenic DNA and could be attributed to an unfavorable distribution of heterology between the Hprt sequences present in the targeting vector and the endogenous Hprt gene.
Type Text
Publisher American Society for Microbiology
Volume 12
Issue 8
First Page 3365
Last Page 3371
Subject Cell Line; DNA Transposable Elements; Embryo; Exons; Mice
Subject MESH DNA; Genetic Vectors; Hypoxanthine Phosphoribosyltransferase; Sequence Homology, Nucleic Acid
Language eng
Bibliographic Citation Mol Cell Biol. 1992 Aug;12(8):3365-71: Deng C, Capecchi MR. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Retrieved on September 22.2006 from http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1321331.
Rights Management Copyright © 1992, American Society for Microbiology. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,463
ARK ark:/87278/s6gt65bz
Setname ir_uspace
ID 702845
Reference URL https://collections.lib.utah.edu/ark:/87278/s6gt65bz