Sedimentation field-flow fractionation: a method for studying particulates in cataractous lens

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Publication Type Journal Article
School or College School of Medicine; College of Science
Department Ophthalmology; Chemistry
Creator Olson, Randall J.; Coldwell, Karin D.; Compton, Bruce J.; Giddings, John C.
Title Sedimentation field-flow fractionation: a method for studying particulates in cataractous lens
Date 1984
Description It is shown that the technique of sedimentation field-flow fractionation (sedimentation [sed] FFF) can be used to determine the particle content and particle size distribution of normal and cataractous lenses. A 31-year-old normal human lens, for example, showed a particle content of 1.5% by weight with diameters ranging from 0.12 micron to 0.9 micron. The urea insoluble material present in the nuclear and cortical fractions from a densely cataractous lens contained particles ranging from 0.12 micron to 1.7 micron, with average sizes of 0.83 micron and 0.82 micron respectively, for the two fractions. These numbers offer a basis for comparison; their actual values may be shifted slightly either up or down depending on the assessment of particle density. These sizes, which correspond to molecular weights of around 2 X 10(9) dalton, are larger than previously reported for lens particulates. The sed FFF method is thus seen to permit fractionation and size analysis of small amounts of lens material in times less than one hour.
Type Text
Publisher Association for Research in Vision and Ophthalmology
Volume 25
Issue 2
First Page 153
Last Page 159
Subject Fractionation; Cataractous Lens; Particulates
Subject MESH Cataract; Particle Size; Chemical Fractionation; Crystallins
Language eng
Bibliographic Citation Caldwell KD, Compton BJ, Giddings JC, Olson RJ. (1984). Sedimentation field-flow fractionation: a method for studying particulates in cataractous lens. Invest Ophthalmol Vis Sci, 25(2), 153-9
Rights Management © Association for Research in Vision and Ophthalmology
Format Medium application/pdf
Identifier ir-main,12746
ARK ark:/87278/s6gb2ng6
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 705459
Reference URL https://collections.lib.utah.edu/ark:/87278/s6gb2ng6