Gene targeting: tapping the cellular telephone.

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Publication Type Journal Article
School or College School of Medicine; College of Science
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Boulet, Anne M.
Title Gene targeting: tapping the cellular telephone.
Date 1990-03-08
Description Gene targeting means that we now have the potential to generate mice of virtually any desired genotype. In the first instance, standards recombinant DNA technology is used to alter a cloned DNA sequence of a chosen locus; the modified potent stem cell derived from a mouse embryo, and homologous recombination between the exogenous and endogenous chromosomal sequence transfers the mutation to the genome. Microinjection of the stem cells containing the modified locus into mouse blastocysts is used to generate germ-line chimaeras. Finally, interbreeding of heterozygous siblings yields animals homozygous for the desired mutation.
Type Text
Publisher Nature Publishing Group
Volume 344
Issue 6262
Subject Animals; Blotting, Southern; Bone and Bones; Gene Targeting; Genotype; Immunochemistry; In Situ Hybridization; Mice
Subject MESH Embryonic and Fetal Development; Esophagus; Genes, Homeobox; Homeodomain Proteins
Language eng
Bibliographic Citation Nature. 1990 Mar 8;344(6262):105. Capecchi MR. Gene targeting: tapping the cellular telephone. Retrieved on November 2, 2006 from
Rights Management copyright ? 1990 Nature Publishing Group. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,567
ARK ark:/87278/s66406xb
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 703298
Reference URL