Identification of androgen response elements in the insulin-like growth factor I upstream promoter

Update Item Information
Publication Type Journal Article
School or College College of Science
Department Physics
Creator Wu, Yong-Shi
Other Author Zhao, Weidong; Zhao, Jingbo; Pan, Jiangping; Wu, Qiaqia; Zhang, Yuanfei; Bauman, William A.; Cardozo, Christopher P.
Title Identification of androgen response elements in the insulin-like growth factor I upstream promoter
Date 2007
Description Testosterone stimulates the expression of IGF-I in cells and tissues that include prostate, muscle and muscle satellite cells, and the uterus. Here, the molecular mechanisms of this effect of testosterone were explored. Testosterone increased IGF-I mRNA levels in HepG2 and LNCaP cells and stimulated the activity of reporter genes controlled by 1.6 kb of the upstream promoter of the human IGF-I gene. An androgen-responsive region that was located between -1320 and -1420 bases upstream of the first codon was identified by truncation studies. The androgen-responsive region was found to contain two sequences resembling known androgen receptor (AR)-binding sites from the Pem1 gene. Reporter genes incorporating these sequences were strongly stimulated by androgens. Each of the androgen-responsive elements (AREs) bound recombinant AR-DNA-binding domain in gel-shift experiments; binding was greatly enhanced by sequences flanking the apparent AR-binding half-sites. Testosterone induced recruitment of AR to sequences of genomic DNA containing these AREs. The two AREs were activated 5-fold more by AR than glucocorticoid receptor. Collectively, these findings indicate the presence of two AREs within the IGF-I upstream promoter that act in cis to activate IGF-I expression. These AREs seem likely to contribute to the up-regulation of the IGF-I gene in prostate tissues, HepG2 cells, and potentially other tissues.
Type Text
Publisher Endocrine Society
Volume 148
Issue 6
First Page 2984
Last Page 2993
Subject IFG-I; Androgen-responsive elements
Subject LCSH Insulin-like growth factor-binding proteins; Somatomedin; Testosterone; Androgen; Human growth -- Endocrine aspects
Language eng
Bibliographic Citation Wu, Y., Zhao, W., Zhao, J., Pan, J., Wu, Q., Zhang, Y., Bauman, W. A., & Cardozo, C. P. (2007). Identification of androgen response elements in the insulin-like growth factor I upstream promoter. Endocrinology, 148(6), 2984-93.
Rights Management (c) Endocrine Society
Format Medium application/pdf
Format Extent 463,212 bytes
Identifier ir-main,9402
ARK ark:/87278/s6417fdh
Setname ir_uspace
ID 704484
Reference URL https://collections.lib.utah.edu/ark:/87278/s6417fdh