OCR Text |
Show SPRING 2013 a Quantitative Analysis of Periprosthetic Collagen Content Around the Percutaneous Osseointegrated Implant ^ord, T.A.; w Jeyapalina, S. l>2 Holt, B.M.; l>2 Beck, J.P.; ^Bloebaum, R.D. 'Bone and Joint Research Laboratory, DVA SLC HCS, Salt Lake City, UT, 84148 'Department of Orthopaedics, University of Utah School of Medicine, Salt Lake City, UT, 84108 'Department of Bioengineering, University of Utah, Salt Lake City, UT, 84112 u Till": UNIVERSITY OF UTAH yj inp*« 2). Ahhoufh It | AHhou|ti this tisiut morphology H In P U H P t U U i A N D M f T H O t n immMiawly for both ImmunoNstochemlcal (IHq and baiK hmoiopal * M A and iubj«rt«S to histoloctcal (valuation. ImmunphlslodwmkaI<IBO a 12 QUANTITATIVE ANALYSIS OF PERIPROSTHETIC COLLAGEN CONTENT AROUNDTHE PERCUTANEOUS OSSEOINTEGRATED IMPLANT Taylor Ford (Sujee Jeyapalina, Brian Holt, Roy Bloebaum) Department of Orthopedics University of Utah Collagen accounts for nearly 3 0 % of all human protein [1 ]. Collagen Type I can be found in healthy skin, tendons, ligaments, and bone; conversely, Type III appears in granulation tissue and is the initial disposition matrix during wound healing [2]. It was proposed that the composition of collagen (ratio 111:1) could be used to measure the healing response around percutaneous implants. In an ongoing animal model, percutaneous titanium implants were coated with biomimetic coatings (keratin and HA) and surgically inserted in the dorsum of six pigs. Periprosthetic tissue samples from this model were collected. Samples underwent two analyses: immunohistochemistry and ELISA. After immunohis-tochemical staining, it appeared that there was more Type III in the granulation tissue at post-necropsy. Additionally, the HA-coating had weaker immunofluorescence signals for Type III in the granulation bed, indicating that the periprosthetic tissue was healing. The data from ELISA analyses of Types I and III found that the concentration ratio of Type 111:1 differed with the bio-mimetic coating types. The keratin-coating showed a ratio of 1:1.40, while the HA-coating showed a ratio of 1:5.11. The control (no coating) demonstrated a ratio of lll:l of 1:0.60. Additionally, the measurement of collagen III in the granulation tissue was compared to the collagen III in the healthy tissue sample. It was evident that the H A coated sample had the most healthy granulation tissue, showing only a 1.9:1 ratio of granulation collagen III to healthy tissue collagen III. Conversely, the keratin coating showed a ratio of 9.1:1 and the control showed a ratio of 8.5:1 for the same measurement. Conclusively, a refined method based on this study could be used as a bio-compatibility assessment tool for future biomaterial selection. |